A5081697244- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Genetic factors in colorectal cancer
- RNA Research and Splicing
- Helicobacter pylori-related gastroenterology studies
- Molecular Biology Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Cancer-related molecular mechanisms research
- Genomic variations and chromosomal abnormalities
- Ferroptosis and cancer prognosis
- Carcinogens and Genotoxicity Assessment
- Autophagy in Disease and Therapy
- Genomics and Chromatin Dynamics
- Acute Lymphoblastic Leukemia research
- Gastric Cancer Management and Outcomes
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Mast cells and histamine
- Protein Tyrosine Phosphatases
- SARS-CoV-2 and COVID-19 Research
- PI3K/AKT/mTOR signaling in cancer
- Metabolism, Diabetes, and Cancer
Harbin Medical University
2015-2024
Second Affiliated Hospital of Harbin Medical University
2015-2024
Ministry of Education of the People's Republic of China
2022-2023
Zhejiang Academy of Medical Sciences
2011
China Medical University
2004
Chinese National Human Genome Center
2004
<h3>Background</h3> Gene amplification is a frequent manifestation of genomic instability that plays role in tumour progression and development drug resistance. It manifested cytogenetically as extrachromosomal double minutes (DMs) or intrachromosomal homogeneously staining regions (HSRs). To better understand the molecular mechanism by which HSRs DMs are formed how they relate to methotrexate (MTX) resistance, we used two model systems MTX-resistant HT-29 colon cancer cell lines harbouring...
Double minute chromosomes (DMs) are a hallmark of gene amplification. The relationship between the formation DMs and amplification DM-carried genes remains to be clarified. human colorectal cancer cell line NCI-H716 malignant primitive neuroectodermal tumor SK-PN-DW known contain many DMs. To examine in cells, we performed Affymetrix SNP Array 6.0 analyses verified regions cells. We identified that were amplified overexpressed Using RNA interference, downregulated seven genes, (NDUFB9,...
Background It has been shown that a deletion in the late cornified envelope (LCE) gene cluster (LCE3C_LCE3B‐del) is associated with susceptibility to psoriasis European populations. However, relationship remains unclear northern Chinese population. Objectives The aim of present study was clarify this association Methods In total, 970 patients and 1064 healthy controls were recruited polymerase chain reaction assay used determine frequency deletion. effect on assessed using SPSS software...
Introduction The ErbB-2.1(TOB1) signaling transducer protein is a tumor-suppressive that actively suppresses the malignant phenotype of gastric cancer cells. Yet, TOB1 negatively regulates activation and growth different immune Understanding expression role in environment crucial to maximize its potential targeted immunotherapy. Methods This study employed multiplex immunofluorescence analysis precisely delineate quantify cells within tissue microarrays. Univariate multivariate Cox analyses...
Previously, we identified 3 overlapping regions showing loss of heterozygosity (LOH, R1–R3 from 11 to 30 cM) on chromosome 17 in 45 primary gastric cancers (GCs). The data indicated the presence tumor suppressor genes (TSGs) involved GC. Among putative TSGs these regions, HIC1 (in SR1) and TOB1 SR3) remain be examined By immunohistochemistry (IHC), methylation-specific PCR (MSP) western blot, evaluated expression regulation status for protein We narrowed down deletion intervals defined five...
Double minutes (DMs) are hallmarks of gene amplification. However, their molecular structure and the mechanisms formation largely unknown. To elucidate underlying mechanism DMs, we obtained cloned DMs using microdissection; degenerated oligonucleotide primed polymerase chain reaction (DOP-PCR) from ovarian cancer cell line UACC-1598. Two large amplicons, 284 kb AmpMYCN, originating locus 2p24.3 391 AmpEIF5A2, 3q26.2, were found co-amplified on same DMs. The two amplicons joined through a...
TOB1, a member of the BTG/TOB protein family, inhibits tumor cell proliferation. We previously observed down-regulation and phosphorylation TOB1 in gastric cancer (GC). Here, we examined subcellular distribution clinical significance expression GC. Immunohistochemical analysis 341 primary GC corresponding normal tissue samples demonstrated that nuclear was lower than (80.4% vs. 92.4%), decreased correlated with high TNM stage. By contrast, higher (66.0% 36.4%), associated poorly...
Abstract Double minute chromosomes ( DMs ) are extrachromosomal cytogenetic structures found in tumour cells. As hallmarks of gene amplification, often carry oncogenes and drug‐resistance genes play important roles malignant progression drug resistance. The mitogen‐activated protein kinase MAPK signalling pathway is frequently dysregulated human tumours, which induces genomic instability, but it remains unclear whether a close relationship exists between . In the present study, we focused on...
Abstract By allelotyping for loss of heterozygosity (LOH), we previously identified a deletion region that harbors the candidate tumor suppressor gene DAL-1 at 18p11.3 in sporadic gastric cancers (GCs). The expression and function GCs remained unclear. Here, demonstrated absence or notable decreases mRNA protein was highly correlated with CpG hypermethylation promoter primary GC tissues cell lines. Furthermore, abnormal subcellular localization also observed cells. Exogenous effectively...
Sei-1 is an oncogene capable of inducing double minute chromosomes (DMs) formation. DMs are hallmarks amplification and contribute to oncogenesis. However, the mechanism formation remains unelucidated.DMs significantly increased during serial passage in vivo gradually decreased following culture vitro. micro nuclei (MN) was found be responsible for reduction. Of DMs-carrying genes, Met markedly amplified, overexpressed highly correlated with Inhibition signaling number reduced genes. We...
Abstract Gene amplification chiefly manifests as homogeneously stained regions (HSRs) or double minutes (DMs) in cytogenetically and extrachromosomal DNA (ecDNA) molecular genetics. Evidence suggests that gene is becoming a hotspot for cancer research, which may be new treatment strategy cancer. DMs usually carry oncogenes chemoresistant genes are associated with progression, occurrence prognosis. Defining the structure of will facilitate understanding mechanism tumorigenesis. In this study,...
Abstract Background Although DHFR gene amplification has long been known as a major mechanism for methotrexate (MTX) resistance in cancer, the early changes and detailed development of are not yet fully understood. Methods We performed genomic, transcriptional proteomic analyses human colon cancer cells with sequentially increasing levels MTX-resistance. Results The genomic evolved three phases (pre-amplification, homogenously staining region (HSR) extrachromosomal DNA (ecDNA)). confirm that...
Abstract The locus at 17q12 erb‐b2 receptor tyrosine kinase 2 (ERBB2) has been heavily amplificated and overexpressed in gastric cancer (GC), but it remains to be elucidated about the clinical significance of co‐amplification co‐overexpression PGAP3 gene located around ERBB2 GC. profile four GC cell lines tissue microarrays containing 418 primary tissues was assessed investigate co‐amplified genes, evaluate impact genes on malignancy Co‐amplification accompanied with observed a haploid...
Background We previously identified the tumor suppressor gene TOB1 as related to gastric cancer. The purpose of this study was explore whether induces autophagy through AKT/mTOR signaling pathway in Methods Western blotting used detect protein levels TOB1, LC3, AKT, mTOR, phosphorylated (p) and p-mTOR. A double fluorescent GFP-RFP-LC3 fusion trace by laser confocal microscopy. Autophagosomes were observed transmission electron Results conversion LC3-I LC3-II LC3-II/LC3-I ratio significantly...
We previously confirmed that transducer of ERBB2, 1 (TOB1) gene, can induce autophagy in gastric cancer cells. Studies have shown the biogenesis exosomes overlaps with different processes, which helps to maintain self-renewal and homeostasis body This study is aimed at verifying whether overexpressing TOB1 induces by secreting cells its underlying mechanisms. Differential ultracentrifugation was used extracted from culture medium cell line AGS-TOB1 ectopically (exo-AGS-TOB1, experimental...
We investigated the association between p53 mRNA expression and clinically relevant surrogates of nucleotide excision repair (ERCC1 XPA) in 28 ovarian cancer specimens. observed that platinum-resistant tumors showed higher levels p53, ERCC1, XPA than platinum-sensitive tumors; patterns responders differed substantially from nonresponders; a strong correlation with tumor tissues, but not tumors. 47% mutations sequence analysis were related to clinical response chemotherapy. conclude influence...
The TOB1 (ErbB-2,1) gene is an anti-proliferative factor that has the potential to regulate cell growth and encodes a member of transducer erbB-2/B-cell translocation protein. association between polymorphisms gastric cancer (GC) risk still unclear. In this study, 506 GC cases 548 healthy controls (HCs) were collected evaluate eleven SNPs (rs35220381, rs12950561, rs7221352, rs61482741, rs9303568, rs34700818, rs12949115, rs9903822, rs12601477, rs11656976 rs4626) in population northeast China....