A5015589509- Language and Culture
- Microbial Metabolic Engineering and Bioproduction
- Diverse Academic Research Studies
- Medical History and Research
- Genetic Associations and Epidemiology
- Historical and Cultural Studies of Poland
- Gene Regulatory Network Analysis
- Polish Historical and Cultural Studies
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Medical and Biological Sciences
- Prostate Cancer Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Biochemical and Molecular Research
- Bacterial Genetics and Biotechnology
- Mycobacterium research and diagnosis
- Protein Structure and Dynamics
- Medicine and Dermatology Studies History
- RNA modifications and cancer
- Tuberculosis Research and Epidemiology
- History of Medicine Studies
- Health, Work, and Social Studies in Poland
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- Genetic factors in colorectal cancer
University of Surrey
2013-2024
University of Zielona Góra
2022
Lasy Państwowe
2022
Wroclaw University of Applied Informatics "Horizon"
2020
Polish Wood Cluster
2019
Simcyp (United Kingdom)
2016-2018
Wroclaw Medical University
2013-2018
Karkonosze College in Jelenia Góra
2017-2018
Bristol-Myers Squibb (United States)
2017
Polish Academy of Sciences
1999-2016
Abstract Breast, ovarian, and prostate cancers are hormone-related may have a shared genetic basis, but this has not been investigated systematically by genome-wide association (GWA) studies. Meta-analyses combining the largest GWA meta-analysis data sets for these totaling 112,349 cases 116,421 controls of European ancestry, all together in pairs, identified at P < 10−8 seven new cross-cancer loci: three associated with susceptibility to (rs17041869/2q13/BCL2L11;...
<h3>Abstract</h3> <h3>Objectives</h3> To develop and validate a genetic tool to predict age of onset aggressive prostate cancer (PCa) guide decisions who screen at what age. <h3>Design</h3> Analysis genotype, PCa status, select single nucleotide polymorphisms (SNPs) associated with diagnosis. These were incorporated into survival analysis estimate their effects on diagnosis (that is, not eligible for surveillance according National Comprehensive Cancer Network guidelines; any Gleason score...
An impediment to the rational development of novel drugs against tuberculosis (TB) is a general paucity knowledge concerning metabolism Mycobacterium tuberculosis, particularly during infection. Constraint-based modeling provides approach investigating microbial but has not yet been applied genome-scale M. tuberculosis. GSMN-TB, metabolic model was constructed, consisting 849 unique reactions and 739 metabolites, involving 726 genes. The calibrated by growing bovis bacille Calmette Guérin in...
The kinetics of prokaryotic gene expression has been modelled by the Monte Carlo computer simulation algorithm Gillespie, which allowed study random fluctuations in number protein molecules during expression. model, when applied to LacZ expression, is good agreement with experimental data. influence frequencies transcription and translation initiation on studied a simulations promoter ribosome binding site effectiveness changed range values reported for various genes. We show that genes...
Abstract Motivation: The availability of a huge amount molecular data concerning various biochemical reactions provoked numerous attempts to study the dynamics cellular processes by means kinetic models and computer simulations. Biochemical frequently involve small numbers molecules (e.g. few transcriptional regulator binding one ‘molecule’ DNA regulatory region). Such are subject significant stochastic fluctuations. Monte Carlo methods must be employed functional consequences fluctuations...
A large number of cDNA inserts were sequenced from a high-quality library chicken bursal lymphocyte cDNAs. Comparisons to public gene databases indicate that the collection represents more than 2,000 new, full-length transcripts. This resource defines structure and coding potential fraction B-cell specific housekeeping genes whose function can be analyzed by disruption in DT40 line.
Mycobacterium tuberculosis infects a third of the world's population. Primary involving active fast bacterial replication is often followed by asymptomatic latent tuberculosis, which characterised slow or non-replicating bacteria. Reactivation infection switch back to can lead post-primary transmissible tuberculosis. Mycobacterial mechanisms involved in growth switching rate provide rational targets for development new drugs against persistent mycobacterial infection. Using chemostat culture...
Streptomycetes sense and respond to the stress of phosphate starvation via two-component PhoR–PhoP signal transduction system. To identify in vivo targets PhoP we have undertaken a chromatin-immunoprecipitation-on-microarray analysis wild-type phoP mutant cultures and, parallel, quantified their transcriptomes. Most (ca. 80%) previously vitro characterized were identified this study among several hundred other putative novel targets. In addition activating genes for scavenging systems was...
Genome-wide association studies (GWAS) have identified numerous common prostate cancer (PrCa) susceptibility loci. We fine-mapped 64 GWAS regions known at the conclusion of iCOGS study using large-scale genotyping and imputation in 25 723 PrCa cases 26 274 controls European ancestry. detected evidence for multiple independent signals 16 regions, 12 which contained additional newly significant associations. A single signal comprising a spectrum correlated variation was observed 39 regions; 35...
Leprosy has afflicted humankind throughout history leaving evidence in both early texts and the archaeological record. In Britain, leprosy was widespread Middle Ages until its gradual unexplained decline between 14th 16th centuries. The nature of this ancient endemic relationship to modern strains is only partly understood. Modern are currently divided into 5 phylogenetic groups, types 0 4, each with strong geographical links. Until recently, European strains, modern, were thought be...
Abstract Background It is quite important to simulate the metabolic changes of a cell in response change culture environment and/or specific gene knockouts particularly for purpose application industry. If this could be done, design can made without conducting exhaustive experiments, and one screen out promising candidates, proceeded by experimental verification select few particular interest. Although several models have so far been proposed, most them focus on pathways. preferred model...
BACKGROUND Polygenic risk scores comprising established susceptibility variants have shown to be informative classifiers for several complex diseases including prostate cancer. For cancer it is unknown if inclusion of genetic markers that so far not been associated with at a genome-wide significant level will improve disease prediction. METHODS We built polygenic in large training set over 25,000 individuals. Initially 65 were selected. After LD pruning additional prioritized based on their...
The Mycobacterium tuberculosis complex includes bovine and human strains of the bacillus, including tuberculosis, bovis BCG vaccine strain. M. has evolved from a tuberculosis-like ancestor is vaccine. pathogens demonstrate distinct differences in virulence, host range metabolism, but role metabolic pathogenicity poorly understood. Systems biology approaches have been used to investigate metabolism not probe between strains. In this study genome scale networks were constructed interrogated,...
Abstract Although genome-wide association studies have identified over 100 risk loci that explain ∼33% of familial for prostate cancer (PrCa), their functional effects on remain largely unknown. Here we use genotype data from 59,089 men European and African American ancestries combined with cell-type-specific epigenetic to build a genomic atlas single-nucleotide polymorphism (SNP) heritability in PrCa. We find significant differences between variants prostate-relevant marks defined normal...
HOX genes are vital for all aspects of mammalian growth and differentiation, their dysregulated expression is related to ovarian carcinogenesis. The aim the current study was establish prognostic value dysregulation as well its role in platinum resistance. potential target proteins through HOX/PBX interaction also explored context gene determined cancer cell lines primary EOCs by QPCR, compared normal epithelium fallopian tube tissue samples. Statistical analysis included one-way ANOVA...
Using dynamic light scattering we show that aqueous super- to undersaturated solutions of NaCl, (NH4)2SO4, and Na-citrate contain submicrometer size clusters at room temperature. The particle distributions deduced by Laplace inversion the spectra two predominant components. smaller components with radii below 1 nm are attributed mixtures solvated ions larger between 50−500 ion clusters. Knowledge mesoscale structure concentrated electrolyte may be necessary describe effective protein−protein...
Chaperone and protease systems play essential roles in cellular homeostasis have vital functions controlling the abundance of specific proteins involved processes such as transcription, replication, metabolism virulence. Bacteria evolved accurate regulatory to control expression function chaperones potentially destructive proteases. Here, we used a combination transcriptomics, proteomics targeted mutagenesis reveal that clp gene regulator (ClgR) Mycobacterium tuberculosis activates...
Abstract Summary: Constraint-based modeling of genome-scale metabolic networks has been successfully used in numerous applications such as prediction gene essentiality and engineering. We present SurreyFBA, which provides constraint-based simulations network map visualization a free, stand-alone software. In addition to basic simulation protocols, the tool also implements analysis minimal substrate product sets, is useful for engineering nutritional requirements complex vivo environments,...
W pracy w skrócie przedstawiono drogi życiowe, zawodowe i naukowe otorynolaryngologów, którzy zdobyli tytuły naukowe, ordynując na terenie Lwowa pierwszych czterech dekadach XX w. oraz innych ośrodkach po zakończeniu II wojny światowej. Byli to: Tadeusz Ceypek (1904-1990) – organizator otolaryngologii Górnym Śląsku Opolskim; Jan Danielewicz (1903-1982) twórca dziecięcej polskiej światowej; Antoni Dobrzański (1893-1953) założyciel Kliniki Otolaryngologicznej powojennej Warszawie; Wiktor...
Objectives: This study focuses on enhancing the efficiency of generating virtual patient populations, a critical component in various Quantitative Systems Pharmacology (QSP) modeling workflows such as clinical trial simulation, digital twin generation, characterizing biological variability, and biomarker generation. The primary objectives are to streamline generation process, reduce computational costs, improve quality generated population. Methods: We developed comprehensive cloud-based QSP...