A5061749338- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Pancreatic function and diabetes
- Antimicrobial Resistance in Staphylococcus
- Advanced biosensing and bioanalysis techniques
- Genetic Associations and Epidemiology
- Parasitic infections in humans and animals
- Microbial Metabolism and Applications
- Enzyme Structure and Function
- DNA and Nucleic Acid Chemistry
- Antifungal resistance and susceptibility
- Monoclonal and Polyclonal Antibodies Research
- Biofuel production and bioconversion
- Biochemical and Molecular Research
- Pharmacogenetics and Drug Metabolism
- CRISPR and Genetic Engineering
- Parasitic Infections and Diagnostics
- Mycobacterium research and diagnosis
- Genomics and Rare Diseases
- Glycosylation and Glycoproteins Research
- Tuberculosis Research and Epidemiology
- Enzyme Catalysis and Immobilization
- Bacterial biofilms and quorum sensing
Universidad Nacional Autónoma de México
2009-2025
National Institute of Genomic Medicine
2013-2022
Instituto de Medicina Genómica
2017-2021
Universidad Autónoma del Estado de Morelos
2012
Secretaria de Salud
2011
Instituto Barretos de Tecnologia
2009
Biotechnology Institute
1999
Instituto de Biotecnología de León
1993
City of Hope
1982
Hope Research Institute
1982
Tapeworms (Cestoda) cause neglected diseases that can be fatal and are difficult to treat, owing inefficient drugs. Here we present an analysis of tapeworm genome sequences using the human-infective species Echinococcus multilocularis, E. granulosus, Taenia solium laboratory model Hymenolepis microstoma as examples. The 115- 141-megabase genomes offer insights into evolution parasitism. Synteny is maintained with distantly related blood flukes but find extreme losses genes pathways...
Mexico harbors great cultural and ethnic diversity, yet fine-scale patterns of human genome-wide variation from this region remain largely uncharacterized. We studied genomic within over 1000 individuals representing 20 indigenous 11 mestizo populations. found striking genetic stratification among populations at varying degrees geographic isolation. Some groups were as differentiated Europeans are East Asians. Pre-Columbian substructure is recapitulated in the ancestry admixed across...
Protein-coding genetic variants that strongly affect disease risk can yield relevant clues to pathogenesis. Here we report exome-sequencing analyses of 20,791 individuals with type 2 diabetes (T2D) and 24,440 non-diabetic control participants from 5 ancestries. We identify gene-level associations rare (with minor allele frequencies less than 0.5%) in 4 genes at exome-wide significance, including a series more 30 SLC30A8 alleles conveys protection against T2D, 12 gene sets, those...
<h3>Importance</h3> Latino populations have one of the highest prevalences type 2 diabetes worldwide. <h3>Objectives</h3> To investigate association between rare protein-coding genetic variants and prevalence in a large population to explore potential molecular physiological mechanisms for observed relationships. <h3>Design, Setting, Participants</h3> Whole-exome sequencing was performed on DNA samples from 3756 Mexican US individuals (1794 with 1962 without diabetes) recruited 1993 2013....
The positively charged amino-terminal region of the signal peptide has been proposed to have an important role at initial step protein secretion across membrane (loop model). To test this hypothesis, charge on prolipoprotein Escherichia coli outer was altered by using synthetic oligonucleotides from +2 +1, 0, and -1 guided site specific mutagenesis a plasmid DNA carrying inducible lipoprotein gene. wild-type sequence sectio, Met-Lys-Ala-Thr-Lys (+2), thus changed Met-Lys-Asp-Thr-Lys (I-1;...
The Cuatro Cienegas basin in the Chihuahuan desert is a system of springs, streams, and pools. These ecosystems support >70 endemic species abundant living stromatolites other microbial communities, representing oasis high biodiversity. Here, we combine data from molecular microbiology geology to document biodiversity this unique environment. Ten water samples locations within two neighboring valleys as well three wet sediments were analyzed. phylogeny prokaryotic populations was determined...
Abstract L ‐phenylalanine ( ‐Phe) is an aromatic amino acid with diverse commercial applications. Technologies for industrial microbial synthesis of ‐Phe using glucose as a starting raw material currently achieve relatively low conversion yield Y Phe/Glc ). The purpose this work was to study the effect PTS (phosphotransferase transport system) inactivation and overexpression different versions feedback inhibition resistant chorismate mutase‐prephenate dehydratase (CM‐PDT) on ) productivity...
Type 2 diabetes (T2D) affects more than 415 million people worldwide, and its costs to the health care system continue rise. To identify common or rare genetic variation with potential therapeutic implications for T2D, we analyzed replicated genome-wide protein coding in a total of 8,227 individuals T2D 12,966 without Latino descent. We identified novel variant IGF2 gene associated ∼20% reduced risk T2D. This variant, which has an allele frequency 17% Mexican population but is Europe,...
Abstract Excretory/Secretory (ES) proteins play an important role in the host-parasite interactions. Experimental identification of ES is time-consuming and expensive. Alternative bioinformatics approaches are cost-effective can be used to prioritize experimental analysis therapeutic targets for parasitic diseases. Here we predicted functionally annotated T. solium genome using integration tools. Additionally, developed a novel measurement evaluate potential antigenicity secretome sequence...
Understanding the genetic structure of Native American populations is important to clarify their diversity, demographic history, and identify factors relevant for biomedical traits. Here, we show a history reconstruction from 12 whole genomes belonging six distinct ethnic groups representing three main described clusters Mexico (Northern, Southern, Maya). Effective population size estimates all remained below 2,000 individuals up 10,000 years ago. The proportion missense variants predicted...
The expression of genes transcribed by the RNA polymerase with alternative sigma factor <r54 (Ecr54) is absolutely dependent on activator proteins that bind to enhancer-like sites, located far upstream from promoter. These unique prokaryotic proteins, known as enhancer-binding (EBP), mediate open promoter complex formation in a reaction NTP hydrolysis. best characterized this family regulators are NtrC and Nif A, which activate required for ammonia assimilation nitrogen fixation,...
The role of the eucaryotic T-A-T-A box (Hogness box)homology sequence located -30 base pairs upstream from RNA initiation site has been further examined by oligodeoxynucleotide-directed site-specific mutagenesis.A method was employed which allows insertion nucleotide-specific mutations into virtually any double-stranded, recombinant plasmid DNA.A synthetic mixed oligonucleotide, bearing defined multiple nucleotide substitutions at a single site, used both as specific mutagen during primed...
A prokaryotic consensus sequence promoter has been chemically synthesized and cloned in bacterial plasmid vectors. This designed is biologically active promotes efficient expression of the genes to which it fused. It an unusually strong vitro, capable specifying multiple rounds transcription even when there a large molar excess heparin present prior addition RNA polymerase. These properties make this useful for vitro production RNAs. 2-base-pair spacer mutant -35 region transversion have...
Background Whole genome sequencing (WGS) has been proposed as a tool for diagnosing drug resistance in tuberculosis. However, reports of its effectiveness endemic countries with important numbers are scarce. The goal this study was to evaluate the procedure isolates from tuberculosis region Mexico. Methods WGS analysis performed 81 positive clinical known phenotypic profile against first-line drugs (isoniazid, rifampin, ethambutol, pyrazinamide and streptomycin). Mutations related were...
Mycobacterium tuberculosis catalase-peroxidase (Mt-KatG) is a bifunctional heme-dependent enzyme that has been shown to activate isoniazid (INH), the widely used antibiotic against (TB). The L333V-KatG variant associated with INH resistance in clinical M. isolates from Mexico. To understand better mechanisms of activation, its catalytic properties (catalase, peroxidase, and IN-NAD formation) crystal structure were compared those wild-type (WT-KatG). rate formation mediated by WT-KatG was 23%...
The COVID-19 pandemic, caused by SARS-CoV-2, has led to significant challenges worldwide, including diverse clinical outcomes and prolonged post-recovery symptoms known as Long COVID or Post-COVID-19 syndrome. Emerging evidence suggests a crucial role of metabolic reprogramming in the infection's long-term consequences. This study employs novel approach utilizing machine learning (ML) explainable artificial intelligence (XAI) analyze alterations patients. Samples were taken from cohort 142...