A5100344239- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Platelet Disorders and Treatments
- Lipid Membrane Structure and Behavior
- Glycosylation and Glycoproteins Research
- Cholesterol and Lipid Metabolism
- Synthesis and Biological Evaluation
- Algal biology and biofuel production
- Complement system in diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Neuropeptides and Animal Physiology
- Telomeres, Telomerase, and Senescence
- Lymphoma Diagnosis and Treatment
- Zebrafish Biomedical Research Applications
- Rice Cultivation and Yield Improvement
- GABA and Rice Research
- Plant Micronutrient Interactions and Effects
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Advanced Data Storage Technologies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Enzyme Structure and Function
- Agricultural Science and Fertilization
- Microfluidic and Capillary Electrophoresis Applications
Center for Excellence in Molecular Cell Science
2015-2025
University of Chinese Academy of Sciences
2017-2025
Xi'an Jiaotong University
2024
Yanbian University Hospital
2021-2024
Chinese Academy of Sciences
2009-2023
Institute of Hydrobiology
2023
Shanghai Jiao Tong University
2023
Hefei Institutes of Physical Science
2022
Institute of Plasma Physics
2022
State Key Laboratory of Plant Genomics
2015-2020
Antigen-triggered T-cell receptor (TCR) phosphorylation is the first signaling event in T cells to elicit adaptive immunity against invading pathogens and tumor cells. Despite its physiological importance, underlying mechanism of TCR remains elusive. Here, we report a key regulating initiation phosphorylation. The major kinase Lck shows high selectivity on four CD3 proteins TCR. CD3ε only chain that can efficiently interact with Lck, mainly through ionic interactions between basic...
T cell receptor (TCR) plays a fundamental role in adaptive immunity, and TCR-T therapy holds great promise for treating solid tumors other diseases. However, there is noticeable absence of chemical tools tuning TCR activity. In our study, we screened natural sterols their regulatory effects on function identified 7-alpha-hydroxycholesterol (7a-HC) as potent inhibitor signaling. Mechanistically, 7a-HC promoted membrane binding CD3ε cytoplasmic domain, crucial signaling component the TCR-CD3...
The mechanisms underlying the functional impairment and metabolic reprogramming of T lymphocytes in multiple myeloma (MM) have not been fully elucidated. In this study, single-cell RNA sequencing was used to compare gene expression profiles cells bone marrow peripheral blood 10 newly diagnosed MM patients versus 3 healthy donors. Unbiased bioinformatics analysis revealed 9 cytotoxic cell clusters. All clusters had higher senescence markers (e.g., KLRG1 CTSW) than control; some...
Although the T-cell-mediated immune response to influenza virus has been studied extensively, little information is available on direct interaction between and T-cells that pertains severe diseases in humans animals. To address these issues, we utilized BALB/c mouse model combined with primary infected A/WSN/33 investigate whether an affinity for vivo. We observed small proportions of CD4(+) CD8(+) spleen thymus expressed viral proteins mice. A significant proportion displayed expression...
This study characterized the cardiac telocyte (TC) population both in vivo and vitro, investigated its telomerase activity related to mitosis.Using transmission electron microscopy a phase contrast microscope, typical morphological features of TCs were observed; by targeting cell surface proteins CD117 CD34, + CD34 sorted via flow cytometry validated immunofluorescence based on primary culture. Then optimized basal nutrient medium for selected was examined with counting kit 8. Under this...
To investigate the molecular mechanism of proteolytic cleavage unusually large von Willebrand Factor(ULVWF) on endothelial cells by ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type 1 repeats-13) in absence fluid shear stress, so as to provide a theoretical basis for pathogenesis thrombotic thrombocytopenic purpura (TTP) other disorders.
A key feature following pathogen recognition by resistance (R) proteins containing NB-ARC (nucleotide-binding adaptor shared Apaf-1, R proteins, and Ced-4) domains is the hypersensitive response (HR). However, underpinning mechanisms integral to this process remain relatively opaque. Here we show that a gain-of-function mutation in protein, RLS1 (Rapid Leaf Senescence1), triggers high-light-dependent HR-like cell death rice. The RLS1-mediated defense independent of SA (salicylic acid)...
Abstract The recombination activating gene (RAG or RAG1/RAG2 complex)-mediated adaptive immune system is a hallmark of jawed vertebrates. It has been reported that RAG originated in invertebrates. However, whether further evolved once it arose vertebrates remains largely unknown. Here, we found zebrafish (zRAG) had lower activity than mouse (mRAG). Intriguingly, the attenuated stability RAG2 (zRAG2), but not RAG1, caused reduced V(D)J efficiency compared to mRAG at 37 °C which are body...
// Li Hua 1 , Mingli Fang 2 Boqi Dong Sheng Guo Cuiyun Cui Jiwei Liu Yun Yao Yue Xiao Xin Yunjia Ren Xiuping Meng Xu Hao Peiyan Zhao Yilan Song Liying Wang Yongli Yu Department of Immunology, College Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, 130021, China Molecular Biology, Correspondence to: Wang, email: wlying@jlu.edu.cn Yu, ylyu@jlu.edu.cn Keywords: NKG2DL, allogeneic tumors, early stage, NK cells, NKG2D + cells Received: October 12,...
To investigate the biological function of Cysteine rich (CysR) domain a disintegrin and metalloprotease with thrombospondin type 1 repeats-13 (ADAMTS13) on cleavage von Willebrand factor (vWF) provide experimental evidence for exploring pathogenesis thrombotic thrombocytopenic purpura (TTP).The six amino acids (EDGTLS) in ADAMTS13 CysR were point mutated one by one, mutant proteins expressed purified. The products vWF polymer wild-type or under denaturing condition shear stress separated 1%...