A5102826277- Glioma Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Acute Ischemic Stroke Management
- Meningioma and schwannoma management
- Systemic Lupus Erythematosus Research
- Neurological Disease Mechanisms and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Anesthesia and Neurotoxicity Research
- Brain Metastases and Treatment
- Vascular Malformations Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Intracranial Aneurysms: Treatment and Complications
- Neurofibromatosis and Schwannoma Cases
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Neurological Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Stroke Rehabilitation and Recovery
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Management of metastatic bone disease
- Hedgehog Signaling Pathway Studies
- Peripheral Neuropathies and Disorders
University Medical Center of the Johannes Gutenberg University Mainz
2015-2024
Johannes Gutenberg University Mainz
2015-2024
University of Applied Sciences Mainz
2024
German Centre for Cardiovascular Research
2021
University of Münster
2007-2017
Focus (Germany)
2014-2017
Medical University of Vienna
1999-2015
University Medical Center
2015
Klinik und Poliklinik für Psychosomatische Medizin und Psychotherapie
2014
Evangelisches Krankenhaus Bielefeld
2011-2013
The discovery of spontaneous neuronal replacement in the adult brain has shifted experimental stroke therapies toward a combined approach preventing cell death and inducing plasticity. Brain-derived neurotrophic factor (BDNF) was shown to induce antiapoptotic mechanisms after reduce infarct size secondary death. Moreover, intact animals, BDNF is potent stimulator neurogenesis.The current study analyzed effects on induction progenitor migration sensorimotor recovery cortical photothrombotic...
T lymphocytes are increasingly recognized as key modulators of detrimental inflammatory cascades in acute ischaemic stroke, but the potential cell-targeted therapy brain ischaemia is largely unexplored. Here, we characterize effect inhibiting leukocyte very late antigen-4 and endothelial vascular cell adhesion molecule-1-mediated invasion-currently most effective strategy primary neuroinflammatory disease murine stroke models. Very blockade by monoclonal antibodies improved outcome models...
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic cytokine responsible for the proliferation, differentiation, and maturation of cells myeloid lineage, which was cloned more than 20 years ago. Here we uncovered novel function GM-CSF in central nervous system (CNS). We identified alpha-receptor as an upregulated gene screen ischemia-induced genes cortex. This receptor broadly expressed on neurons throughout brain together with its ligand induced by ischemic insults....
To evaluate toxicity and efficacy of the combination lomustine, temozolomide (TMZ) involved-field radiotherapy in patients with newly diagnosed glioblastoma (GBM).Thirty-one adult (median Karnofsky performance score 90; median age, 51 years) accrued two centers received (60 Gy 2-Gy fractions) chemotherapy lomustine 100 mg/m2 (day 1) TMZ mg/m2/d (days 2 to 6) individual dose adjustments according hematologic toxicity.A five courses (range, one six courses) were delivered. WHO grade 4...
Abstract Cellular and humoral inflammations play important roles in ischemic brain injury. The effectiveness of immunomodulatory therapies may critically depend on the chosen experimental model. Our purpose was to compare post‐ischemic neuroinflammation among murine permanent transient middle cerebral artery occlusion ( MCAO ) models. Permanent induced by transtemporal electrocoagulation 30 minutes or 90 intraluminal filament C57BL/6 mice. Infiltration leukocyte subpopulations quantified...
Abstract Ependymoma with YAP1‐MAMLD1 fusion is a rare, recently described supratentorial neoplasm of childhood, few cases published so far. We report on 15 pediatric patients ependymomas carrying fusions, their characteristic histopathology, immunophenotype and molecular/cytogenetic, radiological clinical features. The was documented by RT‐PCR/Sanger sequencing, tumor genomes were studied molecular inversion probe (MIP) analysis. Significant copy number alterations identified GISTIC (Genomic...
The HIT-2000-BIS4 trial aimed to avoid highly detrimental craniospinal irradiation (CSI) in children < 4 years of age with nonmetastatic medulloblastoma by systemic chemotherapy, intraventricular methotrexate, and risk-adapted local radiotherapy.From 2001-2011, 87 patients received chemotherapy methotrexate. Until 2006, CSI was reserved for nonresponse or progression. After radiotherapy introduced nonresponders classic (CMB) large-cell/anaplastic (LCA). DNA methylation profiles infantile...
The DNA repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) is a key player in tumor cell resistance. Promoter methylation, MGMT activity and immunohistochemistry are used for determining the status. However, it unclear whether promoter methylation correlates with of pretreatment predicts status recurrences. To address these questions, we determined immunoreactivity recurrent glioblastomas (GB, WHO Grade IV), astrocytomas (WHO III). We show that GB were methylated display range...
Oncolytic virotherapy is a potential treatment modality under investigation for various malignancies including malignant brain tumors. Unlike some other natural or modified viruses that show oncolytic activity against cerebral neoplasms, the rodent parvovirus H-1 (H-1PV) completely apathogenic in humans. H-1PV efficiently kills number of tumor cells without harm to corresponding normal ones. In this study, concept H-1PV-based glioma was tested rat (RG-2 cell-derived) and human (U87 gliomas...
The contribution of neuroinflammation and specifically brain lymphocyte invasion is increasingly recognised as a substantial pathophysiological mechanism after stroke. FTY720 potent treatment for primary neuroinflammatory diseases by inhibiting circulation immigration. Previous studies using transient focal ischemia models showed protective effect but did only partially characterize the involved pathways. We tested neuroprotective properties in permanent cortical analyzed underlying...
The DNA repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) causes resistance of cancer cells to alkylating agents and, therefore, is a well-established predictive marker for high-grade gliomas that are routinely treated with drugs. Since MGMT highly epigenetically regulated, the promoter methylation status taken as an indicator silencing, predicting outcome glioma therapy. usually determined by specific PCR (MSP), which labor intensive and error-prone method often used...
First-line drug in the treatment of glioblastoma, most severe brain cancer, is temozolomide (TMZ), a DNA-methylating agent that induces critical damage O6-methylguanine (O6MeG). This lesion cytotoxic through generation mismatch repair-mediated DNA double-strand breaks (DSBs), which trigger apoptotic pathways. Previously, we showed O6MeG also cellular senescence (CSEN). Here, show TMZ-induced CSEN late response has similar kinetics to apoptosis, but at fourfold higher level. cells high amount...
Abstract Inclusion body myositis (IBM) is the most prevalent inflammatory muscle disease in older adults with no effective therapy available. In contrast to other myopathies such as subacute, immune-mediated necrotizing myopathy (IMNM), IBM follows a chronic course both and degenerative features of pathology. Moreover, causal factors molecular drivers progression are largely unknown. Therefore, we paired single-nucleus RNA sequencing spatial transcriptomics from patient biopsies map...
Hyperbaric oxygenation (HBO) after focal cerebral ischemia reduces infarct size and improves outcome when applied early stroke. Here, we evaluated effects of HBO on permanent magnetic resonance imaging (MRI) monitoring to study lesion evolution.Rats underwent middle artery occlusion (MCAO). Two hours later, animals were treated with (100% O(2)/2 atm; n=17) for 1 hour or room air (n=17). Animals serial MRI studies (DWI, PI, T2) beginning 90 minutes MCAO. Neuroscore was assessed (5-point...
Background and Purpose— Both application of neurotrophic factors like brain-derived factor (BDNF) constraint-induced movement therapy forced arm use have been shown to potentially improve outcome after stroke. The aim the present study was check whether postischemic long-term correlates specific modifications in abundance various neurotransmitter receptors. Methods— Adult male Wistar rats were subjected photothrombotic ischemia assigned treatment groups (n=5 each) with end points at 3 6...
Hyperbaric oxygen (HBO) and normobaric hyperoxia (NBO) protect the brain parenchyma cerebral microcirculation against ischemia. We studied their effect on secondary hemorrhage after thrombolysis in two thromboembolic middle artery occlusion (MCAO) (tMCAO) models. Beginning 60 minutes tMCAO with either thrombin-induced thromboemboli (TT) or calcium-induced (CT), spontaneously hypertensive rats ( n=96) breathed air, 100% O 2 (NBO), at 3 bar for 1 hour. Immediately thereafter, recombinant...
The neuroprotective potential of citicoline in acute ischemic stroke has been shown many experimental studies and, although the exact mechanisms are still unknown, a clinical Phase III trial is currently underway. Our present study was designed to check whether also enhances neuroregeneration after stroke.Forty Wistar rats were subjected photothrombotic and treated either with daily injections (100 mg/kg) or vehicle for 10 consecutive days starting 24 hours ischemia induction. Sensorimotor...