A5101640482- Immune Response and Inflammation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Plant tissue culture and regeneration
- Corneal Surgery and Treatments
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- T-cell and B-cell Immunology
- Retinoids in leukemia and cellular processes
- Ferroptosis and cancer prognosis
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Computational Drug Discovery Methods
- Inflammation biomarkers and pathways
- Plant nutrient uptake and metabolism
- bioluminescence and chemiluminescence research
- Advances in Cucurbitaceae Research
- 14-3-3 protein interactions
- Virology and Viral Diseases
- Plant Virus Research Studies
- Seaweed-derived Bioactive Compounds
Xiamen Medical College
2021-2024
Institute of Zoology
2024
State Key Laboratory of Stem Cell and Reproductive Biology
2024
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2024
Wenzhou Medical University
2024
Zhejiang DongFang Vocational and Technical College
2024
Union Hospital
2023
Huazhong University of Science and Technology
2023
Hunan University of Traditional Chinese Medicine
2022
Obstetrics and Gynecology Hospital of Fudan University
2022
Dendritic cells (DC) are migratory that exhibit complex trafficking properties in vivo. The present study was designed to characterize receptor expression and responsiveness chemoattractants of human DC obtained from PBMC by culture with granulocyte/macrophage-CSF IL-13. expressed appreciable levels the CCR1, CCR2, CCR5 receptors for CC chemokines chemokine CXCR1, CXCR2, CXCR4. increased intracellular free calcium migrated response MCP-3, MCP-4, RANTES, MIP-1alpha, MIP-1beta, MIP-5/HCC2 CXC...
Abstract Under certain physiological and pathological conditions, naturall killer (NK) cells rapidly accumulate in tissues. Chemokines are an essential component of the current paradigm leukocyte recruitment. The present study was designed to investigate responsiveness NK prototypic C‐C chemokine, monocyte chemotactic protein‐1 (MCP‐1). MCP‐1 induced migration across filters interleukin (IL)‐2‐activated cells, whereas it a weak attractant for unstimulated cells. Maximal induction required...
N6-methyladenosine (m6A) has emerged as the most prevalent post-transcriptional modification on mRNA that contributes prominently to tumorigenesis. However, specific function of m6A methyltransferase methyltransferase-like 3 (METTL3) in colorectal cancer (CRC) remains elusive. Herein, we explored biological METTL3 CRC progression. Clinically, was frequently upregulated tissues, cell lines, and plasma samples its high expression predicted poor prognosis patients. Functionally, knockdown...
The intestinal epithelial repair after injury is coordinated by stem cells (ISCs). Fucosylation catalyzed fucosyltransferase 2 (FUT2) of the epithelium beneficial to mucosal healing but poorly defined influence on ISCs. dextran sulfate sodium (DSS) and lipopolysaccharide (LPS) model were used assess role FUT2 ISCs injury. apoptosis, function, stemness analyzed using organoids from WT Fut2ΔISC (ISC-specific Fut2 knockout) mice incubated with LPS fucose. N-glycoproteomics, UEA-1...
Objective: To measure the frequency and associated factors of cervicovaginal HIV shedding to determine impact sexually transmitted disease (STD) treatment on shedding. Design: Cross-sectional study with 1-week follow-up. Setting: Confidential clinic for female sex workers in Abidjan, Côte d'lvoire. Participants: A total 1201 workers. Interventions: STD based clinical signs. Main outcome measures: serostatus; at enrolment follow-up; status Results: Cervicovaginal HIV-1 HIV-1-seropositive...
The transcription factor Woolly (Wo) and its downstream gene CycB2 have been shown to regulate trichome development in tomato (Solanum lycopersicum). It has demonstrated that only the gain-of-function allele of Slwo (SlWoV, woolly motif mutant allele) can increase density; however, it remains unclear why two alleles function differently development. In this study, we used Nicotiana benthamiana as a model cloned homologues SlCycB2 (named Nbwo NbCycB2). We also constructed with same mutation...
The subcortical maternal complex (SCMC) is essential for safeguarding female fertility in mammals. Assembled oocytes, the SCMC maintains cleavage of early embryos, but underlying mechanism remains unclear. Here, we report that 14-3-3, a multifunctional protein, component SCMC. By resolving structure 14-3-3-containing SCMC, discover phosphorylation TLE6 contributes to recruitment 14-3-3. Mechanistically, during maternal-to-embryo transition, stabilizes 14-3-3 protein and proper control...
This study aimed to identify the different expression of microRNAs (miRNAs) in plasma derived exosomes patients with esophageal squamous cell carcinoma (ESCC).A total 9 ESCC and benign diseases were involved. miRNA sequencing was performed screen differential between controls. The function on proliferation migration abilities determined by CCK-8 analysis, wound scratch transwell test. Predicted target genes screened databases confirmed RT-qPCR.We identified a 10 miRNAs (7 upregulated 3...
The human endometrium is a highly regenerative tissue that believed to have two main types of stem cells: endometrial mesenchymal/stromal cells (eMSCs) and epithelial (eESCs). So far, eMSCs been extensively studied, whereas the studies eESCs are constrained by inability culture expand them in vitro. aim this study establish an efficient method for production from potential clinical application intrauterine adhesion (IUA).Here we developed condition with combination some small molecules vitro...
Abstract Dendritic cell (DC) migration to secondary lymphoid organs is a crucial step initiate adaptive immune responses. This requires the expression of functional CCR7 chemokine receptor on DC undergoing maturation. Here, we show that natural retinoid 9-cis retinoic acid (9cRA) and synthetic fenretinide (4-HPR) specifically inhibit up-regulation maturing human DCs, without affecting early steps As consequence, mature DCs do not migrate in vitro toward CCL19. Importantly, 4-HPR 9cRA by...
The purpose is to investigate the mechanism of subconjunctival fibrosis caused by benzalkonium chloride (BAC), which most common preservative in ophthalmic preparations.The left eyes male Sprague-Dawley rats were topically treated with 0.01% BAC or PBS twice daily for 1 month. Primary conjunctival fibroblasts (CFs) exposed 24 hours 0.00005% BAC, 0.000075% + LY2157299 (a selective transforming growth factor β receptor type I inhibitor); NS-398 cyclooxygenase-2 inhibitor) and PBS,...
Purpose.: To investigate the exact mechanism by which keratocytes promote corneal neovascularization. Methods.: The expression of matrix metalloproteinase 13 (MMP13), cluster differentiation 146 (CD146), VEGFa, VEGFc, VEGF receptor (r)2, and VEGFr3 normal alkali-burned rat corneas was determined via quantitative (q)RT-PCR and/or Western blot analysis or in situ hybridization. Corneal neovascularization observed under a slit lamp microscope evaluated immunohistochemistry. cells that expressed...
Gastric cancer (GC) is the third leading cause of cancer-related death worldwide, partially due to lack effective screening strategies. Thus, there a stringent need for non-invasive biomarkers improve patient diagnostic efficiency in GC.This study initially filtered messenger RNAs (mRNAs) as prospective through bioinformatics analysis. Clinical validation was conducted using circulating mRNA plasma from patients with GC. Relationships between expression levels target genes and...
Panicle degradation causes severe yield reduction in rice. There are two main types of panicle degradation: apical spikelet abortion and basal degeneration. In this study, we isolated characterized the mutant ( asa ), which exhibits degeneration defects spikelets. This had a pleiotropic phenotype, by reduced plant height, increased tiller number, decreased pollen fertility. Map-based cloning revealed that OsASA encodes boric acid channel protein showed highest expression inflorescence,...
3086 Background: In clinical trials certain Hsp90 inhibitors, including AUY922, SNX-5422, and 17-DMAG have caused visual symptoms suggesting retinal dysfunction; others, ganetespib 17-AAG, not. Previous animal toxicology experiments suggested that changes may be linked to photoreceptor degeneration or cell death. Here histopathologic and/or exposure profiles of inhibitors with without reports ocular toxicity were evaluated understand the observed differences in profile between agents this...
Abstract Background: The epigenetic modification of LncRNA N6-methyladenosine (m6A) methylation plays a wide role in the invasion and metastasis various malignant tumors. However, LncRNA-ATB m6A colorectal cancer (CRC) has not been systematically elucidated. Methods: Detect regulatory effect ALKBH5 on by Luciferin reporter gene. expression was detected IHC qPCR CRC tissues cells. Transwell scratch tests were used to detect overexpression after knockdown replacement migration ability Western...