A5035121757- Renal Transplantation Outcomes and Treatments
- Cytomegalovirus and herpesvirus research
- Organ Transplantation Techniques and Outcomes
- Herpesvirus Infections and Treatments
- Renal Diseases and Glomerulopathies
- SARS-CoV-2 and COVID-19 Research
- Neonatal Health and Biochemistry
- Blood groups and transfusion
- Blood donation and transfusion practices
- Parvovirus B19 Infection Studies
- Transplantation: Methods and Outcomes
- Retinal and Optic Conditions
- T-cell and B-cell Immunology
- Complement system in diseases
- Hemoglobinopathies and Related Disorders
- Peripheral Neuropathies and Disorders
- Hematopoietic Stem Cell Transplantation
- Monoclonal and Polyclonal Antibodies Research
- Polyomavirus and related diseases
- Pregnancy and Medication Impact
- Iron Metabolism and Disorders
- Animal Virus Infections Studies
- Reproductive System and Pregnancy
- COVID-19 Impact on Reproduction
- Immune Cell Function and Interaction
University of Lübeck
2013-2025
University Hospital Schleswig-Holstein
2012-2025
Institute for Transfusion Medicine
2011-2019
Universitäres Kinderwunschzentrum Lübeck
2017
German Red Cross
2011-2013
Medizinische Hochschule Hannover
2013
University Hospital Münster
2011
University Medical Center
2006-2011
Institute of Immunology
2006
RWTH Aachen University
1992
The novel coronavirus SARS-CoV-2 causes the infectious disease COVID-19. Newly developed mRNA vaccines can prevent spread of virus. Headache is most common neurological symptom in over 50% those vaccinated. Detailed information about clinical characteristics this form headache has not yet been described. aim study to examine detail headaches occurring after vaccination against COVID-19 with BNT162b2 vaccine for first time. In a multicentre observational cohort study, data on features and...
Background/Objectives: In this ongoing, multicenter, global cohort observational study, phenotypes of headaches after COVID-19 vaccination were directly compared between different vaccines. Methods: Phenotypes postvaccinal headache recorded in 18,544 participants. The study was launched immediately the start campaign on 12 January 2021 and continued until 1 August 2023. Specific aspects related variables collected via an online questionnaire. clinical characteristics patients vaccinated with...
The human leukocyte antigen (HLA) complex contains the most polymorphic genes in genome. classical HLA class I and II define specificity of adaptive immune responses. Genetic variation at is associated with susceptibility to autoimmune infectious diseases plays a major role transplantation medicine immunology. Currently, are characterized using Sanger- or next-generation sequencing (NGS) limited amplicon repertoire labeled oligonucleotides for allele-specific sequences. High-quality...
The prognostic value of preformed donor-specific HLA antibodies (DSA), which are only detectable by sensitive methods, remains controversial for kidney transplantation.The outcome 4233 consecutive transplants performed between 2012 and 2015 in 18 German transplant centers was evaluated. Most used a stepwise pretransplant antibody screening with bead array tests differentiation positive samples single antigen assays. Using these results, DSA against HLA-A, -B, -C, -DRB1 -DQB1 were determined....
To determine the prevalence and impact on patient outcome of active human cytomegalovirus infections in patients with prolonged treatment an intensive care unit.Retrospective analysis stored plasma samples.Anesthesiological unit a university hospital.All 138 treated for at least 14 days (of total 4940 admitted during study period). Immunocompromised inconclusive results DNA were excluded.None.Stored samples stay tested DNA. Sixty-four 255 evaluable from 99 immunocompetent DNA-positive mean...
The most frequently reported neurological adverse event of ChAdOx1 nCoV-19 (AZD1222) vaccine is headache in 57.5%. Several cases cerebral venous thrombosis (CVT) have developed after vaccination. Headache the leading symptom CVT. For differential diagnosis headaches attributed to this and CVT, it central clinical importance whether and, if so, how phenotypes course these can be differentiated. study aims examine detail phenotype vaccine.
BACKGROUND: Human cytomegalovirus (CMV) is considered to latently infect blood cells. Transfusion‐transmitted infection (TT‐CMV) of immunocompromised patients occurs despite the use CMV‐seronegative or leukoreduced units. STUDY DESIGN AND METHODS: The prevalence CMV DNA in plasma was investigated 82 donors who had previously been seronegative for and showed anti‐CMV immunoglobulin G first time, 598 were seropositive at least 1 year, 150 donors. In a second part study, overall donations...
In Brief BACKGROUND: Positive end-expiratory pressure (PEEP) during mechanical ventilation may impose different degrees of stress on healthy lungs. On the assumption that is reflected by cytokine production, we performed a translational study investigating effect PEEP bronchoalveolar and systemic mediator levels in isolated perfused mouse lungs (IPL) patients with METHODS: (Part I) IPL were ventilated pressures 0, 3, 6, or 10 cm H2O end-inspiratory (EIP) 25 H2O. Interleukin (IL)-6 macrophage...
Background and Objectives As cytomegalovirus (CMV) DNA is frequently detectable in the plasma of recently infected sero-positive blood donors, information concerning primary CMV infection important for identification possibly infectious donors. Materials Methods Monitoring 17 982 donors antibodies identified 14 subjects with ongoing infection. Thirteen were interrogated possible sources CMV-related symptoms, monitored antigens, plasma, leucocytes urine, course IgG IgM as well markers...
BACKGROUND: Capillary hemoglobin (Hb) measurement before admission for whole blood donation is performed in many services, spite of several studies reporting donors with low Hb values being missed by capillary screening. STUDY DESIGN AND METHODS: Predonation and venous levels 9209 first‐time presenting were compared. testing was conducted photometric determination finger‐stick samples a method (Donor Checker, HemoCue) using hematology analyzer samples. RESULTS: Both measurements available...
Background Donors with short interdonation intervals (e.g., apheresis donors) have an increased risk of window period donations. The frequency cytomegalovirus ( CMV ) donations is important information to decide whether selection seronegative donors might be advantageous for patients at transfusion‐transmitted infections TT‐CMV ). Study Design and Methods seroconversion in 93 positive results routine antibody testing within most 35 days after the last sample was evaluated by W estern blot...
Heterologous vaccinations against SARS-CoV-2 with ChAdOx1 nCoV-19 and a second dose of an mRNA-based vaccine have been shown to be more immunogenic than homologous nCoV-19. In the current study, we examined kinetics antibody response three different vaccination regimens (homologous vs. + BNT162b2 or mRNA-1273) in longitudinal manner; whether there are differences latency amplitude early which markers most suitable detect these responses.We performed assays for anti-S1 IgG IgA, anti-NCP...
SARS-CoV-2 serosurveillance is important to adapt infection control measures and estimate the degree of underreporting. Blood donor samples can be used as a proxy for healthy adult population. In repeated cross-sectional study from April 2020 2021, September April/May 2022, 13 blood establishments collected 134,510 anonymised specimens donors in 28 regions across Germany. These were tested antibodies against spike protein nucleocapsid, including neutralising capacity. Seroprevalence was...
Background C ytomegalovirus ( CMV ) DNA is frequently detected in plasma of newly seropositive donors. Selection leukoreduced blood products from donors with remote infection could avoid transfusion‐transmitted infections TT ‐ due to primarily infected However, there are no data about the prevalence reactivations long‐term compared incidence window period donations seronegative Therefore, optimal transfusion strategy for at‐risk patients unclear. Study Design and Methods Whole samples 22,904...
Background. Preformed donor-specific HLA antibodies (DSA) are a well-known risk factor in kidney transplantation. There is still considerable debate, however, about the optimal stratification among patients with preformed DSA. Additionally, data on prognostic value of different crossmatch assays DSA-positive scarce. Methods. living transplant recipients were selected from multicenter study examining 4233 consecutive renal transplants. An additional 7 2 further centers included. Flow...
The assignment of human leucocyte antigens (HLAs) against which antibodies are detected as unacceptable (UAGs) avoids allocation HLA- incompatible allografts. There is uncertainty to what extent UAGs decrease the probability receiving a kidney offer.Kidney transplantations in 3264 patients on waiting lists six German transplant centres were evaluated for period at least 2 years. proportion excluded offers due was calculated virtual panel-reactive (vPRAs).In common Eurotransplant Kidney...
Background: Due to findings on adverse reactions and clinical efficacy of different vaccinations against SARS-CoV-2, the administration vaccination regimens containing both adenoviral vector vaccines mRNA-based has become common. Data are still needed direct comparison immunogenicity for these regimens. Methods: We compared markers (anti-S1 IgG/IgA, neutralizing antibodies, T-cell response) with three (homologous ChAdOx1 nCoV-19 (n = 103), or mixture mRNA-1273 116) BNT162b2 105)) at two time...
Abstract Background and Objectives Serologic typing with monoclonal anti‐D is mandatory for RHD antigen determination before transfusion, but due to aberrant (weak or partial) variants of RHD, results may be ambiguous molecular RHD‐typing required. Before that, RHD‐negative (RHD –) red blood cells concentrates (RBCs) shall transfused avoid formation, which probably leads wastage ‐ RBCs. Study Design Methods All patients in serologic were assessed retrospectively. The proportions at risk...
Abstract HLA antibody screening is conducted routinely prior to kidney transplantation, but the comparative prognostic value and cost‐effectiveness of different methods are unclear. Pre‐transplant sera 141 patients transplanted between 1998 2000 were screened by ELISA Luminex assays, specificities reactive determined using bead array techniques. detected donor‐specific antibodies ( DSA ) in 19 patients, who had a higher incidence impaired graft function (60% vs. 20%, p = 0.04)...
SUMMARY Objective and Background To assess the performance characteristics of two fully automated human cytomegalovirus ( HCMV ) antibody tests. Materials Methods Samples from negatively or not pre‐screened blood donors were tested by Biotest anti‐ recombinant IgG enzyme‐linked immunosorbent assay ELISA in comparison to Abbott Architect CMV [chemiluminescent microparticle immunoassay CMIA )]. For clarification, samples with discordant results between both assays subjected supplemental...
Delayed graft function (DGF) occurs in a significant proportion of deceased donor kidney transplant recipients and was associated with injury inferior clinical outcome. The aim the present multi-center study to identify immunological non-immunological predictors DGF determine its influence on outcome presence absence human leukocyte antigen (HLA) antibodies. 1,724 patients who received during 2008–2017 whom pre-transplant serum sample available were studied. Graft survival first 3...