A5051022862- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Immune Response and Inflammation
- Diabetes and associated disorders
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Dermatology and Skin Diseases
- NF-κB Signaling Pathways
- Diabetes Management and Research
- Advanced Biosensing Techniques and Applications
- Nanoplatforms for cancer theranostics
- Adrenal Hormones and Disorders
- Estrogen and related hormone effects
- Cytomegalovirus and herpesvirus research
- Animal Nutrition and Physiology
- Rabbits: Nutrition, Reproduction, Health
- Comparative constitutional jurisprudence studies
- Peroxisome Proliferator-Activated Receptors
- Pancreatic function and diabetes
- Latin American socio-political dynamics
- Chemokine receptors and signaling
Reaction Biology (Germany)
2022-2025
University of Freiburg
2019-2024
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2024
The University of Queensland
2023-2024
Agriculture and Food
2024
Hospital Universitario Austral
2023
Medizinische Hochschule Hannover
2020
Center for Experimental and Clinical Infection Research
2014-2020
University of Buenos Aires
2010-2019
Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society
2012-2016
Abstract Autoimmunity can occur when a checkpoint of self-tolerance fails. The study familial autoimmune diseases reveal pathophysiological mechanisms involved in more common diseases. Here, by whole-exome/genome sequencing we identify heterozygous, autosomal-dominant, germline loss-of-function mutations the SOCS1 gene ten patients from five unrelated families with early onset manifestations. intracellular protein is known to downregulate cytokine signaling inhibiting JAK-STAT pathway....
Summary Type 1 diabetes (T1DM) is a T cell-mediated autoimmune disease that selectively destroys pancreatic β cells. The only possible cure for T1DM to control autoimmunity against cell-specific antigens. We explored whether the natural compound curcumin, with anti-oxidant and anti-inflammatory activities, might down-regulate cell response cells improve outcome in diabetes. employed two accelerated models: (i) cyclophosphamide (CYP) administration non-obese diabetic (NOD) mice (ii) adoptive...
The Nck-associated protein 1–like (NCKAP1L) gene, alternatively called hematopoietic 1 (HEM-1), encodes a lineage–specific regulator of the actin cytoskeleton. Nckap1l-deficient mice have anomalies in lymphocyte development, phagocytosis, and neutrophil migration. Here we report, for first time, NCKAP1L deficiency cases humans. In two unrelated patients Middle Eastern origin, recessive mutations abolishing expression led to immunodeficiency, lymphoproliferation, hyperinflammation with...
While antibiotics are intended to specifically target bacteria, most known affect host cell physiology. In addition, some antibiotic classes reported as immunosuppressive for reasons that remain unclear. Here, we show Linezolid, a ribosomal-targeting (RAbo), effectively blocked the course of T cell-mediated autoimmune disease. Linezolid and other RAbos were strong inhibitors helper-17 effector function in vitro, showing this effect was independent their activity. Perturbing mitochondrial...
Upon antigen‐specific or allogeneic activation, T cells sharply increase their metabolic activity to cope with augmented needs for proliferation and effector functions. Therefore, enzymes involved in energy metabolism constitute attractive targets modulate the of pathogenic setting graft‐versus‐host‐disease (GVHD). Here, we show that deficient acetyl‐CoA carboxylase 1 (TACC1) are dramatically less than wild‐type (WT) a lethal C57BL/6 into BALB/c model acute GVHD permitted sustained survival...
The identification and characterization of rare immune cell populations in humans can be facilitated by their growth advantage the context specific genetic diseases. Here, we use autoimmune lymphoproliferative syndrome to identify a population FAS-controlled TCRαβ+ T cells. They include CD4+, CD8+, double-negative cells defined CD38+CD45RA+T-BET− expression pattern. These unconventional are present healthy individuals, generated before birth, enriched lymphoid tissue, do not expand during...
Abstract Macrophages play a critical role in tumor development by shaping the microenvironment. In particular, M2-polarized macrophage phenotype is associated with pro-tumoral functions such as promoting angiogenesis, immune suppression, and progression. Consequently, numerous therapeutic approaches aim to repolarize M2 macrophages mitigate their tumor-supportive behavior. While these effects are consistently effectively demonstrated vitro our laboratory, translating findings into vivo...
Abstract The identification and quantification of biomarkers in clinical blood samples play a pivotal role personalized medicine, enabling disease monitoring therapeutic assessment. Protein phosphorylation, key post-translational modification, reflects cellular signaling immune or tumor cells. To exploit this, we developed platform detecting phosphorylation states proteins from via flow cytometry. Our approach analyzes phosphorylated STAT5 (pSTAT5), ERK1/2 (pERK1/2), p38 (p-p38), vital...
Compound A (CpdA) is a dissociating non-steroidal glucocorticoid receptor (GR) ligand which has anti-inflammatory properties exerted by down-modulating proinflammatory gene expression. By favouring GR monomer formation, CpdA does not enhance (GC) response element-driven expression, resulting in reduced side effect profile as compared to GCs. Considering the importance of Th1/Th2 balance final outcome immune and inflammatory responses, we analyzed how selective modulation differentially...
Abstract Background One of the main roles intestinal mucosa is to protect against environmental hazards. Supplementation xylo-oligosaccharides (XOS) known selectively stimulate growth beneficial bacteria and improve gut health function in chickens. XOS may have an impact on integrity epithelia where cell turnover critical maintain compatibility between digestive barrier functions. The aim study was evaluate effect arabinoxylan-rich fraction (AXRF) supplementation epithelial broiler Methods A...
Abstract Dendritic cells (DC) initiate the adaptive immune response. Glucocorticoids (GCs) down-modulate function of DC. Compound A (CpdA, (2-(4-acetoxyphenyl)-2-chloro-N-methyl-ethylammonium chloride) is a plant-derived GR-ligand with marked dissociative properties. We investigated effects CpdA on in vitro generated GM-CSF-conditioned bone marrow-derived DC (BMDC). CpdA-exposed BMDC exhibited low expression cell-surface molecules and diminution release proinflammatory cytokines upon LPS...
The NF-κB transcription factor subunit RelB is important for the full activation of conventional dendritic cells (cDCs) during T-cell-dependent immune responses. Although number splenic DCs greatly reduced in RelBnull mice, cause and consequences this deficiency are currently unknown. To circumvent impact pleiotropic defects mice we used a reporter model expression (RelBKatushka mice) conditionally deleted (RelBCD11c-Cre mice). Thereby, can show here that essential differentiation CD117+...
Background: Compound A (CpdA) is a dissociating non-steroidal glucocorticoid receptor (GR) ligand which has antiinflammatory properties exerted by down-modulating proinflammatory gene expression.By favouring GR monomer formation, CpdA does not enhance (GC) response element-driven expression, resulting in reduced side effect profile as compared to GCs.Considering the importance of Th1/Th2 balance final outcome immune and inflammatory responses, we analyzed how selective modulation...
Glucocorticoid (GCs) hormones have pleiotropic activities in the body playing important roles metabolism and modulating/regulating stress immune responses. Upon stimuli that trigger or inflammatory responses there is a concomitant activation of hypothalamus-pituitary-adrenal axis ultimately manifested by an increase synthesis release GCs to systemic circulation. play pivotal role interface between neuroendocrine systems modulating final outcome response. The successful resolution response...
Abstract CAR T cells are donor/patient-derived genetically engineered to express a chimeric receptor against specific tumor antigen (TA), which allows an MHC-independent recognition and targeting of cells. While CART cell therapies have been tremendously successful hematological cancers, with 6 products being approved up date from the FDA (Sengsayadeth S etal, EJHaem. 2022), activity on solid tumors is more difficult. Solid remain unique challenge in part due trafficking problems cells,...