A5101994532- Signaling Pathways in Disease
- Transplantation: Methods and Outcomes
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Lung Cancer Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Organ and Tissue Transplantation Research
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Tracheal and airway disorders
- Cancer-related gene regulation
- Lung Cancer Research Studies
- COVID-19 Clinical Research Studies
- Heat shock proteins research
- Organ Transplantation Techniques and Outcomes
- Mesenchymal stem cell research
- Neurogenetic and Muscular Disorders Research
- Medical Imaging and Pathology Studies
- Muscle metabolism and nutrition
- Gut microbiota and health
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- SARS-CoV-2 and COVID-19 Research
Wuxi People's Hospital
2010-2025
Nanjing Medical University
2011-2025
Air Force Medical University
2016-2025
Beijing University of Chinese Medicine
2024
Xijing Hospital
2017-2024
Jinan University
2017-2020
Xian Yang Central Hospital
2019
Center for Translational Molecular Medicine
2018
Brigham and Women's Hospital
2017-2018
Function Promoting Therapies (United States)
2017-2018
Abstract In face of the everlasting battle toward COVID-19 and rapid evolution SARS-CoV-2, no specific effective drugs for treating this disease have been reported until today. Angiotensin-converting enzyme 2 (ACE2), a receptor mediates virus infection by binding to spike protein. Although ACE2 is expressed in lung, kidney, intestine, its expressing levels are rather low, especially lung. Considering great infectivity COVID-19, we speculate that SARS-CoV-2 may depend on other routes...
Abstract Ferroptosis is a nonapoptotic cell death process that requires cellular iron and the accumulation of lipid peroxides. In progressive rheumatoid arthritis (RA), synovial fibroblasts proliferate abnormally in presence reactive oxygen species (ROS) elevated oxidation. Here we show, using collagen-induced (CIA) mouse model, imidazole ketone erastin (IKE), ferroptosis inducer, decreases fibroblast numbers synovium. Data from single-cell RNA sequencing further identify two groups have...
<h3>Importance</h3> The Institute of Medicine set the recommended dietary allowance (RDA) for protein at 0.8 g/kg/d entire adult population. It remains controversial whether intake greater than RDA is needed to maintain anabolism in older adults. <h3>Objective</h3> To investigate increasing 1.3 adults with physical function limitations and usual within improves lean body mass (LBM), muscle performance, function, fatigue, well-being augments LBM response a anabolic drug. <h3>Design, Setting,...
SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape, compromising the effectiveness of existing vaccines neutralizing antibodies. An in-depth investigation on COVID-19 pathogenesis is urgently needed develop a strategy against variants. Here, we identified CD147 as universal receptor for its Meanwhile, Meplazeumab, humanized anti-CD147 antibody, could block cellular entry variants-alpha, beta, gamma, delta, with inhibition rates 68.7, 75.7, 52.1, 62.3% at 60...
Recent evidence suggests that CD147 serves as a novel receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Blocking via anti-CD147 antibody could suppress the in vitro SARS-CoV-2 replication. Meplazumab is humanized IgG
Clear cell renal carcinoma (ccRCC) is the most common histologic type of RCC. However, spatial and functional heterogeneity immunosuppressive cells mechanisms by which their interactions promote immunosuppression in ccRCC have not been thoroughly investigated. To further investigate cellular regional ccRCC, we analyzed single-cell transcriptome RNA sequencing data from four patients, were obtained samples multiple regions, including tumor core, tumor-normal interface, distal normal tissue....
Background. Primary graft dysfunction (PGD) develops within 72 h after lung transplantation (Lung Tx) and greatly influences patients’ prognosis. This study aimed to establish an accurate machine learning (ML) model for predicting grade 3 PGD (PGD3) Lung Tx. Methods. retrospective incorporated 802 patients receiving Tx between July 2018 October 2023 (640 in the derivation cohort 162 external validation cohort), 640 were randomly assigned training internal cohorts a 7:3 ratio. Independent...
Abstract Background Gut microbiome on predicting clinical responses to immune checkpoint inhibitors (ICIs) has been discussed in detail for decades, while microecological features of the lower respiratory tract within advanced non‐small‐cell lung cancer (NSCLC) are still relatively vague. Methods During this study, 26 bronchoalveolar lavage fluids (BALF) from NSCLC participants who received inhibitor monotherapy were performed 16S rRNA sequencing and untargeted metabolome identify...
The pathogen Pseudomonas aeruginosa uses quorum sensing (QS) to control virulence and biofilm formation. Enzymatic disruption of is a promising anti-infection therapeutic strategy that does not rely on antibiotics. Here, novel gene (aii810) encoding an N-acylhomoserine lactonase was isolated from the Mao-tofu metagenome for first time. Aii810 encoded protein 269 amino acids expressed in Escherichia coli BL21 (DE3) soluble form. It showed highest activity at 20°C, it maintained 76.5% 0°C more...
Meplazumab, a humanized CD147 antibody, has shown favourable safety and efficacy in our previous clinical studies. In DEFLECT (NCT04586153), 167 patients with severe COVID-19 were enroled randomized to receive three dosages of meplazumab placebo. Meplazumab at 0.12 mg/kg, compared the placebo group, showed benefits significantly reducing mortality by 83.6% (2.4% vs. 14.6%, p = 0.0150), increasing proportion alive discharged without supplemental oxygen (82.9% 70.7%, 0.0337) who achieved...
Protein post-translational modifications (PTMs) are at the heart status of cellular signaling events and broadly involved in tumor progression. CD147 is a biomarker with various PTMs, promoting metastasis metabolism reprogramming. Nevertheless, relationship between PTMs apoptosis has not been reported. In our study, we produced specific anti-CD147-K71 di-methylation (CD147-K71me2) antibody by immunizing di-methylated peptide observed that level CD147-K71me2 non-small cell lung cancer (NSCLC)...
The potential pathogenic mechanism of idiopathic pulmonary fibrosis is widely recognized to involve immune dysregulation. However, the current pool studies has yet establish a unanimous agreement regarding correlation between various types cells and IPF.
Lung cancer is the most common malignant tumor and leading cause of cancer-related deaths worldwide. Because current treatments for advanced non-small cell lung (NSCLC), prevalent histological subtype, show limited efficacy, screening tumor-associated biomarkers using bioinformatics reflects hope to improve early diagnosis prognosis assessment. In our study, a Gene Expression Omnibus dataset was analyzed identify genes with prognostic significance in NSCLC. Upon comparison matched normal...
Abstract Thymic involution during aging is a major cause of decreased T-cell production and reduced immunity. Here, we show that the loss CD147 on T cells prevents thymic senescence, resulting in slowed shrinkage thymus with age increased naive cells. This phenotype result slowing epithelial–mesenchymal transition (EMT) process epithelial (TECs), which eventually leads to adipocyte accumulation. In an vitro coculture system, found TGFβ important factor EMT TECs it can reduce expression...
Immune checkpoint blockade (ICB) only works well for a certain subset of patients with non-small cell lung cancer (NSCLC). Therefore, biomarkers patient stratification are desired, which can suggest the most beneficial treatment.In this study, three datasets (GSE126044, GSE135222, and GSE136961) immunotherapy from Gene Expression Omnibus (GEO) database were analyzed, seven intersected candidates extracted as potential ICB followed by validation The Cancer Genome Atlas (TCGA) dataset in-house...
Extracellular vesicles (EVs) are cell-released of submicrometer size. EVs contain a tissue-specific signature wherein variety proteins and nucleic acids selectively packaged. Recent studies validate that can be used for cancer diagnostics, staging, treatment monitoring. EV-related clinical translation requires effective EV isolation as prerequisite. However, lengthy procedures, low yield, throughput, high levels contaminants disqualify the existing approaches large-scale use. Hence, new...
Abstract Immunosuppression is a major hallmark of tumor progression in non‐small cell lung cancer (NSCLC). Cluster differentiation 147 (CD147), an important pro‐tumorigenic factor, closely linked to NSCLC immunosuppression. However, the role CD147 di‐methylation immunosuppressive microenvironment (TME) remains unclear. Here, at Lys148 (CD147‐K148me2) identified as common post‐translational modification (PTM) that significantly associated with unsatisfying survival outcomes among sufferers,...
Proteomic-based approaches for biomarker discovery are promising strategies used in cancer research. In this study, we performed quantitative proteomic analysis on 16 paired samples of non-small cell lung (NSCLC) and adjacent non-tumor tissues using label-free proteomics liquid chromatography-tandem mass spectrometry/mass spectrometry (LC-MS/MS) to identify differentially expressed proteins. A total 91 proteins were NSCLC compared with among 4047 identified (fold change > 1.5 or < 0.67, P...
Abstract Regulatory T cell (Treg) stability is necessary for the proper control of immune activity and tissue homeostasis. However, it remains unclear whether Treg must be continually reinforced or established during development under physiological conditions. Foxp3 has been characterized as a central mediator genetic program that governs stability. Here, we demonstrate to maintain protein expression, Tregs require cell-to-cell contact, which mediated by CD147-CD98 interaction. As are...
CD147 is highly expressed on the surface of numerous tumor cells to promote invasion and metastasis. Targeting these with CD147-specific antibodies has been validated as an effective approach for lung liver cancer therapy. In immune system, recognized a co-stimulatory receptor impacts outcome thymic selection. Using T cell-specific deletion, we showed here that in thymus indispensable stable αβ cell lineage commitment: loss biases both multipotent DN (double negative) fully committed DP...
Background Lung cancer is a common malignant tumor that threatens human life and associated with high morbidity mortality rates. Calreticulin (CALR) antigen characteristic of immunogenic cell death in non-small lung (NSCLC), which closely related to anti-tumor immunity, but its specific mechanism immunity remains unclear. Methods Immunohistochemical staining was performed detect the expression CALR dendritic cell-lysosome-associated membrane glycoprotein (DC-LAMP) NSCLC tissues. The...