A5050568205- Acute Lymphoblastic Leukemia research
- Genetic factors in colorectal cancer
- Gastrointestinal Tumor Research and Treatment
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Acute Myeloid Leukemia Research
- Oral health in cancer treatment
- Gut microbiota and health
- Cancer Cells and Metastasis
- Colorectal Cancer Treatments and Studies
- Systemic Lupus Erythematosus Research
- Inflammatory Bowel Disease
- Pancreatic and Hepatic Oncology Research
- T-cell and B-cell Immunology
- Gastric Cancer Management and Outcomes
- Hematopoietic Stem Cell Transplantation
- Pregnancy and Medication Impact
- Immune cells in cancer
- Mechanisms of cancer metastasis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Congenital limb and hand anomalies
- Parasitic Infections and Diagnostics
- Hedgehog Signaling Pathway Studies
- Thyroid Cancer Diagnosis and Treatment
Sichuan University
2017-2025
West China Hospital of Sichuan University
2022-2025
State Key Laboratory of Biotherapy
2018-2024
Xuzhou Central Hospital
2023
Abstract Cancer-associated fibroblasts (CAFs) are the predominant components of tumor microenvironment (TME) and influence cancer hallmarks, but without systematic investigation on their ubiquitous characteristics across different types. Here, we perform pan-cancer analysis 226 samples 10 solid types to profile TME at single-cell resolution, illustrating commonalities/plasticity heterogenous CAFs. Activation trajectory major CAF is divided into three states, exhibiting distinct interactions...
Clinical staining and targeting CREB3L1 facilitates precise stratification a potential treatment strategy for thyroid cancer.
Abstract Signet-ring cell carcinoma (SRCC) has specific epidemiology and oncogenesis in gastric cancer, however, with no systematical investigation for prognostic genomic features. Here we report a systematic conducted 1868 Chinese cancer patients indicating that signet-ring cells content was related to multiple clinical characteristics treatment outcomes. We thus perform whole-genome sequencing on 32 pairs of SRC samples, identify frequent CLDN18-ARHGAP26/6 fusion (25%). With 797 additional...
As a widely used anticancer and immunosuppressive agent, methotrexate (MTX) can induce multiple adverse drug reactions (ADRs), such as gastrointestinal toxicity, the mechanisms are poorly understood. Gut microbiota has been reported to be associated with onset of diseases well treatment outcomes different drugs. In this study, mucosal injury was observed in MTX-treated mice, leading significant changes macrophages (i.e., M1/M2 ratio, P < 0.05) but not dendritic cells. Moreover, population,...
Objective The systemic spread of colorectal cancer (CRC) is dominated by the portal system and exhibits diverse patterns metastasis without systematical genomic investigation. Here, we evaluated evolution CRC with multiorgan metastases using multiregion sequencing. Design Whole-exome sequencing was performed on multiple regions (n=74) matched primary tumour, adjacent non-cancerous mucosa, liver lung from six patients CRC. Phylogenetic reconstruction evolutionary analyses were used to...
Early intervention in rheumatoid arthritis (RA) is critical for optimal treatment, but initiation of pharmacotherapy to prevent damage remains unsatisfactory currently. Manipulation the gut microbiome and microbial metabolites can be effective protecting against RA. Thus, probiotics utilized explore new strategies preventing joint damage. The aim this study was mechanisms by which Bifidobacterium pseudocatenulatum affects Based on 16S rRNA sequencing UPLC-MS/MS assays, we focused bile acid...
Abstract As a widely used cancer drug, carboplatin often results in serious side effects, such as gut toxicity. In this study, we examined the effects of microbiota on mice with carboplatin-induced intestinal mucosal damage. Carboplatin resulted mucositis, indicated by weight loss, diarrhoea, and infiltration inflammatory cells. It markedly increased expression cytokines/chemokines intestine. also altered diversity composition microbiota. A significantly higher abundance Prevotella copri (...
Adenomatous polyposis coli (APC) is an important tumor suppressor and mostly linked to the regulation of Wnt/β-catenin signaling pathway. APC mutation has been identified as early event in more than 80% sporadic colorectal cancers (CRCs). Moreover, prognostic differences are observed CRC patients with mutations. Although previous genomics studies have investigated roles concomitant gene mutations determining phenotypic heterogeneity APC-mutant tumors, valuable determinants for still lacking....
Numerous patients with rheumatoid arthritis (RA) manifest severe syndromes, including elevated synovial fluid volumes (SF) abundant immune cells, which can be controlled by TNF/JAK inhibitors. Here, we apply single-cell RNA sequencing (scRNA-seq) and subsequent validations in SF from RA patients. These analyses of tissue show reduced density SF-derived pathogenic cells (e.g., SPP1+ macrophages CXCL13+CD4+ T cells), altered gene expression SPP1 STAT1), molecular pathway changes JAK/STAT),...
The gut microbiota crucially regulates the efficacy of immune checkpoint inhibitor (ICI) based immunotherapy, but underlying mechanisms remain unclear at single-cell resolution. Using RNA sequencing and subsequent validations, we investigate microbiota-ICI synergy by profiling tumor microenvironment (TME) elucidating critical cellular interactions in mouse models. Our findings reveal that intact combined with ICIs may synergistically increase proportions CD8+, CD4+, γδ T cells, reduce...
Abstract B-cell targeted therapy is effective for autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis (RA), although there are setbacks in RA clinical trials. In this study, we designed a novel activating factor (BAFF) antagonist: BAFF-Trap, recombinant glycoprotein with BAFF-binding domains of two BAFF receptors (TACI Br3) linked to Fc domain human IgG1. Unlike TACI-Fc, BAFF-Trap bound but not APRIL (a proliferation-inducing ligand), significantly suppressed...
Abstract Long non‐coding RNAs (lncRNAs) can crucially regulate activation and transformation of cancer‐associated fibroblasts (CAFs) but have not been systematically investigated at single cell resolution. Here, by utilizing integrated single‐cell sequencing datasets, we screened the aberrantly expressed lncRNAs in CAFs, which are major component tumor microenvironment. Our findings revealed a consistent CAF‐specific downregulation Maternally Expressed Gene 3 (MEG3) expression increased MEG3...
GATA3 polymorphisms were reported to be significantly associated with susceptibility of pediatric B-lineage acute lymphoblastic leukemia (ALL), by impacting on expression. We noticed that ALL-related polymorphism located around in the tissue-specific enhancer, and Although regulatory network has been well T cells, functional status is poorly understood B-ALL. thus conducted genome-wide gene expression association analyses reveal genes pathways nine independent B-ALL patient cohorts. In...
Germline variants of PIP4K2A impact susceptibility acute lymphoblastic leukemia (ALL) through inducing its overexpression. Although limited reports suggested the oncogenic role in cancers, regulatory network and prognostic effect this gene remains poorly understood tumorigenesis leukemogenesis. In study, we conducted genome-wide expression association analyses pediatric B-ALL cohorts to discover associated genes pathways, which is followed by bioinformatics investigate related multiple...
Average nucleotide identity (ANI) is a prominent approach for rapidly classifying archaea and bacteria by recruiting both whole genomic sequences draft assemblies. To evaluate the feasibility of ANI in virus taxon demarcation, 685 poxviruses were assessed. Prior to analysis, fragment length threshold value optimized as 200 bp 98 per cent, respectively. After analysis network visualization, resulting sixty-one species (ANI rank) clustered largely consistent with groupings found National...
Introduction Immature ovarian teratomas are a type of malignant germ cell tumor composed complicated types and characterized by pathological features immature neuroectodermal tubules/rosettes. However, there is lack understanding patient-derived (PDT) at the single level. Moreover, whether stem lines derived from (CDT) can be used as models for research on PDT remains to elucidated. Methods Single-cell RNA sequencing (scRNA-seq) subsequent bioinformatic analysis was performed three samples...
Gastrointestinal stromal tumor (GIST) is a common sarcoma of gastrointestinal tract (GIT) with high metastatic and recurrence rates, but the proteomic features are still less understood. Here we performed systematic quantitative proteome profiling GIST from 13 patients classified into very low/low, intermediate risk subgroups. An extended cohort (n = 131) was used for immunohistochemical validation proteins interest. In total, 9177 were quantified, covering 55.9% GIT transcriptome The Human...
Objective Gut dysbiosis contributes to multiple autoimmune diseases, including ankylosing spondylitis, which is commonly treated with tumor necrosis factor (TNF)-α inhibitors (TNFis). Because host TNF-α levels are considered interact gut microbiota, we aimed systematically investigate the microbiota profile of spondylitis patients anti-TNF-α-based treatment and identify potential key bacteria. Methods Fecal samples were collected from 11 healthy controls 24 before/after anti-TNF-α treatment,...
Abstract A significant proportion of hepatocellular carcinoma (HCC) is pathologically associated with hepatitis B virus (HBV) infection, followed by unsatisfied clinical outcomes. The increasing unmet need for HBV‐associated (HBV‐HCC) treatment drives to deeper understand the role intricate immune microenvironment, tumor cell plasticity and dynamics evolution in hepatic carcinogenesis. Thus, a comprehensive understanding cross‐talk between HBV, host cells, microenvironment fundamental...
Background: Tumor metastasis commonly affects pleura in advanced lung cancer and results malignant pleural effusion (MPE). MPE is related to poor prognosis, but without systematic investigation on different cell types their crosstalk at single resolution. Methods: We conducted single-cell RNA-sequencing (scRNA-seq) of patients with effusion. Next, our data were integrated 5 datasets derived from individuals under normal, non-malignant disease carcinomatous conditions. Mesothelial cells...
A small proportion of patients with acute lymphoblastic leukemia (ALL) may experience severe leukopenia after treating 6-mercaptopurine (6MP), which can be largely explained by germline variants in TPMT and NUDT15. However, a minority who suffered such adverse drug reaction have NUDT15 wt/wt genotype, indicating that other genetic factors take part in. In this study, we genotyped 539 exon-located nonsilent pharmacogenetic genes involved phase I/II metabolism 173 pediatric ALL conducted...
Brachydactyly type A1 (BDA1) is an autosomal dominant inherited disease characterized by the shortness/absence of middle phalanges, which can be induced mutations in Indian hedgehog gene ( IHH ). Rheumatoid arthritis (RA) a chronic, systemic autoimmune joint destruction, synovitis, and presence autoantibodies. In this study, proband was diagnosed with both BDA1 RA. We performed whole-exome sequencing four-generation Chinese family to investigate their causal mutation BDA1. A novel in-frame...