Ming Ta Michael Lee
A5088017777- Cancer Genomics and Diagnostics
- Genetic Associations and Epidemiology
- Osteoarthritis Treatment and Mechanisms
- Cancer-related molecular mechanisms research
- Bioinformatics and Genomic Networks
- Genomics and Rare Diseases
- RNA modifications and cancer
- Nutrition, Genetics, and Disease
- BRCA gene mutations in cancer
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Pancreatic function and diabetes
- Evolution and Genetic Dynamics
- Epigenetics and DNA Methylation
- Diabetes Treatment and Management
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Genomic variations and chromosomal abnormalities
- Ovarian function and disorders
- Health, Environment, Cognitive Aging
- Lipoproteins and Cardiovascular Health
- Genetic factors in colorectal cancer
- Gene expression and cancer classification
- Liver Disease Diagnosis and Treatment
- Computational Drug Discovery Methods
Institute of Biomedical Sciences, Academia Sinica
2012-2023
China Medical University
2013-2023
Geisinger Health System
2016-2022
Geisinger Neuroscience Institute
2019-2021
Geisinger Medical Center
2019-2020
Harvard University
2020
Carolina Musculoskeletal Institute
2020
Boston Children's Hospital
2020
RIKEN Center for Integrative Medical Sciences
2013-2018
Prostate Cancer Research
2016
Osteoarthritis affects over 300 million people worldwide. Here, we conduct a genome-wide association study meta-analysis across 826,690 individuals (177,517 with osteoarthritis) and identify 100 independently associated risk variants 11 osteoarthritis phenotypes, 52 of which have not been the disease before. We report thumb spine differences in genetic effects between weight-bearing non-weight-bearing joints. sex-specific early age-at-onset loci. integrate functional genomics data from...
<b>Objective</b> To evaluate the use of prospective screening for HLA-B*58:01 allele to identify Taiwanese individuals at risk severe cutaneous adverse reactions (SCARs) induced by allopurinol treatment. <b>Design</b> National cohort study. <b>Setting</b> 15 medical centres in different regions Taiwan, from July 2009 August 2014. <b>Participants</b> 2926 people who had an indication treatment but not taken previously. Participants were excluded if they undergone a bone marrow transplant, Han...
The aim of this study was to evaluate, for the first time, differences in gene expression profiles normal and osteoarthritic (OA) subchondral bone human subjects. Following histological assessment integrity overlying cartilage severity abnormality by micro-computed tomography, we isolated total RNA from regions interest OA (n = 20) non-OA 5) knee lateral tibial (LT) medial (MT) plateaus. A whole-genome profiling performed on an Agilent microarray platform analyzed using GeneSpring GX11.5....
Abstract Cancer evolves by mutation, with somatic reactivation of retrotransposons being one such mutational process. Germline retrotransposition can cause processed pseudogenes, but whether this occurs somatically has not been evaluated. Here we screen sequencing data from 660 cancer samples for acquired pseudogenes. We find 42 events in 17 samples, especially non-small cell lung (5/27) and colorectal (2/11). Genomic features mirror those germline LINE element retrotranspositions, frequent...
Abdominal aortic aneurysm (AAA) is an important cause of cardiovascular mortality; however, its genetic determinants remain incompletely defined. In total, 10 previously identified risk loci explain a small fraction AAA heritability.We performed genome-wide association study in the Million Veteran Program testing ≈18 million DNA sequence variants with (7642 cases and 172 controls) veterans European ancestry independent replication up to 4972 99 858 controls. We then used mendelian...
Back pain is a common and debilitating disorder with largely unknown underlying biology. Here we report genome-wide association study of back using diagnoses assigned in clinical practice; dorsalgia (119,100 cases, 909,847 controls) intervertebral disc (IDD) (58,854 922,958 controls). We identify 41 variants at 33 loci. The most significant (OR
Single nucleotide polymorphisms (SNPs) are the most common sequence variation in human genome; they have been successfully used mapping disease genes and more recently studying population genetics cancer genetics. In a population-based association study using high-density oligonucleotide arrays for whole-genome SNP genotyping, we discovered that genomes of unrelated Han Chinese, 34 out 515 (6.6%) individuals contained long contiguous stretches homozygosity (LCSHs), ranging size from 2.94 to...
Mitochondrial genomes are separated from the nuclear genome for most of cell cycle by double membrane, intervening cytoplasm, and mitochondrial membrane. Despite these physical barriers, we show that somatically acquired mitochondrial-nuclear fusion sequences present in cancer cells. Most occur conjunction with intranuclear genomic rearrangements, features fragments indicate nonhomologous end joining and/or replication-dependent DNA double-strand break repair dominant mechanisms involved....
Phenome-wide association studies (PheWASs) have been a useful tool for testing associations between genetic variations and multiple complex traits or diagnoses. Linking PheWAS-based phenotypes variant genomic region into network provides new way to investigate cross-phenotype associations, it might broaden the understanding of architecture that exists diagnoses, genes, pleiotropy. We created from one largest PheWASs on electronic health record (EHR)-derived across 38,682 unrelated samples...
Polycystic ovary syndrome is the most common endocrine disorder affecting women of reproductive age. A number criteria have been developed for clinical diagnosis polycystic syndrome, with Rotterdam being inclusive. Evidence suggests that significantly heritable, and previous studies identified genetic variants associated diagnosed using different criteria. The widely adopted electronic health record system provides an opportunity to identify patients studies.To novel under same phenotype...
Conjugation of polyethylene glycol (PEG) to therapeutic molecules can improve bioavailability and efficacy. However, some healthy individuals have pre-existing anti-PEG antibodies certain patients develop antibody during treatment with PEGylated medicines, suggesting that genetics might play a role in PEG immunogenicity. Here we perform genome-wide association studies for IgM IgG responses Han Chinese 177 140 individuals, defined as positive responses, respectively, 492 subjects without...
Antiplatelet response to clopidogrel shows wide variation, and poor is correlated with adverse clinical outcomes. CYP2C19 loss‐of‐function alleles play an important role in this response, but account for only a small proportion of variability clopidogrel. An aim the International Clopidogrel Pharmacogenomics Consortium (ICPC) identify other genetic determinants pharmacodynamics response. A genomewide association study (GWAS) was performed using DNA from 2,750 European ancestry individuals,...
Polygenic risk scores (PRS) derived from European individuals have reduced portability across global populations, limiting their clinical implementation at worldwide scale. Here, we investigate the performance of a wide range PRS models four ancestry groups (Africans, Europeans, East Asians, and South Asians) for 14 conditions high-medical interest. To select best-performing model per trait, first compared performances publicly available scores, constructed new using different methods...
In recent years, the translation of genomic discoveries into mainstream medical practice and public health has gained momentum, facilitated by advent new technologies. However, there are often major discrepancies in pace implementation medicine between developed developing/resource-limited countries. The main reason does not only lie limitation resources but also slow adoption findings poor understanding potential that this discipline offers to rationalize diagnosis treatment. Here, we...
Osteoarthritis (OA) is a common joint disorder with increasing impact in an aging society. While genetic and transcriptomic analyses have revealed some genes non-coding loci associated to OA, the pathogenesis remains incompletely understood. Chromatin profiling, which provides insight into gene regulation, has not been reported OA mainly due technical difficulties. Here, we employed Assay for Transposase-Accessible high throughput sequencing (ATAC-seq) map accessible chromatin landscape...
Abstract Subchondral bone plays a key role in the development of osteoarthritis, however, epigenetics subchondral has not been extensively studied. In this study, we examined genome-wide DNA methylation profiles from three regions on tibial plateau representing disease progression using HumanMethylation450 BeadChip to identify associated alterations. Significant differential methylated probes (DMPs) and genes (DMGs) were identified intermediate late stages during transition stage OA bone....