Amisha Singapuri
A5019862059- Asthma and respiratory diseases
- Long-Term Effects of COVID-19
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory and Cough-Related Research
- Intensive Care Unit Cognitive Disorders
- COVID-19 and Mental Health
- Inhalation and Respiratory Drug Delivery
- Advanced Chemical Sensor Technologies
- COVID-19 Clinical Research Studies
- Eosinophilic Esophagitis
- IL-33, ST2, and ILC Pathways
- Frailty in Older Adults
- Neuroinflammation and Neurodegeneration Mechanisms
- Respiratory Support and Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Sleep and related disorders
- Delphi Technique in Research
- Pediatric health and respiratory diseases
- Health Systems, Economic Evaluations, Quality of Life
- Stroke Rehabilitation and Recovery
- Cystic Fibrosis Research Advances
- Helicobacter pylori-related gastroenterology studies
- Tryptophan and brain disorders
- Diabetes and associated disorders
- Lung Cancer Diagnosis and Treatment
NIHR Leicester Biomedical Research Centre
2014-2025
University of Leicester
2015-2024
Glenfield Hospital
2010-2024
University Hospitals of Leicester NHS Trust
2013-2024
National Institute for Health Research
2019-2023
NIHR Leicester Respiratory Biomedical Research Unit
2013-2021
University of Oxford
2014
The impact of COVID-19 on physical and mental health employment after hospitalisation with acute disease is not well understood. aim this study was to determine the effects COVID-19-related employment, identify factors associated recovery, describe recovery phenotypes. Post-hospitalisation (PHOSP-COVID) a multicentre, long-term follow-up adults (aged ≥18 years) discharged from hospital in UK clinical diagnosis COVID-19, involving an assessment between 2 7 months discharge, including detailed...
No effective pharmacological or non-pharmacological interventions exist for patients with long COVID. We aimed to describe recovery 1 year after hospital discharge COVID-19, identify factors associated patient-perceived recovery, and potential therapeutic targets by describing the underlying inflammatory profiles of previously described clusters at 5 months discharge.
Few genetic studies that focus on moderate-to-severe asthma exist. We aimed to identity novel variants associated with asthma, see whether previously identified for all types of contribute and provide mechanistic insights using expression analyses in patients asthma.In this genome-wide association study, we used a two-stage case-control design. In stage 1, genotyped patient-level data from two UK cohorts (the Genetics Asthma Severity Phenotypes [GASP] initiative the Unbiased BIOmarkers...
The relationship between airway inflammation and obesity in severe asthma is poorly understood.We sought to determine the sputum mediator profiles distribution of eosinophilic people with asthma.Clinical parameters eight mediators were assessed 131 subjects from a single center categorized into lean, overweight, obese groups defined by their body mass index. In an independent group (n = 45) healthy control 19) was enumerated bronchial submucosa, blood, related index.Sputum IL-5 geometric...
Rationale: Shared symptoms and genetic architecture between coronavirus disease (COVID-19) lung fibrosis suggest severe acute respiratory syndrome 2 (SARS-CoV-2) infection may lead to progressive damage. Objectives: The UK Interstitial Lung Disease Consortium (UKILD) post–COVID-19 study interim analysis was planned estimate the prevalence of residual abnormalities in people hospitalized with COVID-19 on basis risk strata. Methods: PHOSP–COVID-19 (Post-Hospitalization COVID-19) used capture...
Post-COVID cognitive deficits, including 'brain fog', are clinically complex, with both objective and subjective components. They common debilitating, can affect the ability to work, yet their biological underpinnings remain unknown. In this prospective cohort study of 1,837 adults hospitalized COVID-19, we identified two distinct biomarker profiles measured during acute admission, which predict outcomes 6 12 months after COVID-19. A first profile links elevated fibrinogen relative...
One in ten severe acute respiratory syndrome coronavirus 2 infections result prolonged symptoms termed long disease (COVID), yet phenotypes and mechanisms are poorly understood
<h3>Background</h3> The genetic basis for developing asthma has been extensively studied. However, association studies to date have mostly focused on mild moderate disease and risk factors severe remain unclear. <h3>Objective</h3> To identify common variants affecting susceptibility asthma. <h3>Methods</h3> A genome-wide study was undertaken in 933 European ancestry individuals with based Global Initiative Asthma (GINA) criteria 3 or above 3346 clean controls. After standard quality control...
Asthma heterogeneity is multidimensional and requires additional tools to unravel its complexity. Computed tomography (CT)-assessed proximal airway remodeling air trapping in asthmatic patients might provide new insights into underlying disease mechanisms.The aim of this study was explore novel, quantitative, CT-determined asthma phenotypes.Sixty-five 30 healthy subjects underwent detailed clinical, physiologic characterization quantitative CT analysis. Factor cluster analysis techniques...
Rationale: Asthma is characterized by disease within the small airways. Several studies have suggested that forced oscillation technique–derived resistance at 5 Hz (R5) − 20 (R20) a measure of airway disease; however, there has been limited validation this measurement to date.Objectives: To validate use R5 R20 as narrowing in asthma, and investigate role plays asthma.Methods: Patient-based complete conducting models were generated from computed tomography scans simulate impact different...
Background The impact of COVID-19 on physical and mental health, employment following hospitalisation is poorly understood. Methods PHOSP-COVID a multi-centre, UK, observational study adults discharged from hospital with clinical diagnosis involving an assessment between two- seven-months later including detailed symptom, physiological biochemical testing. Multivariable logistic regression was performed for patient-perceived recovery age, sex, ethnicity, body mass index (BMI),...
<h2>Summary</h2><h3>Introduction</h3> The multiorgan impact of moderate to severe coronavirus infections in the post-acute phase is still poorly understood. We aimed evaluate excess burden abnormalities after hospitalisation with COVID-19, their determinants, and explore associations patient-related outcome measures. <h3>Methods</h3> In a prospective, UK-wide, multicentre MRI follow-up study (C-MORE), adults (aged ≥18 years) discharged from hospital following COVID-19 who were included Tier...
Post-COVID syndrome involves prolonged symptoms with multi-system and functional impairment lasting at least 12 weeks after acute COVID-19. We aimed to determine the efficacy of exercise-based rehabilitation interventions, either face-to-face or remote, compared usual care in individuals experiencing following a hospitalisation This single-blind randomised controlled trial two COVID interventions (face-to-face remote) participants hospitalisation. The were remote eight-week program...
Cellular and computational models bronchial biopsies from asthma patients show that a DP 2 antagonist reduces airway smooth muscle mass in asthma.
Precision medicine has spurred new innovations in molecular pathology leading to recent advances the analysis of exhaled breath as a non-invasive diagnostic tool. Volatile organic compounds (VOCs) detected have potential reveal wealth chemical and metabolomic information. This study describes development method for breath, based on automated thermal desorption (TD) combined with flow modulated comprehensive two-dimensional gas chromatography (GC×GC) dual flame ionisation quadrupole mass...
Acute cardiorespiratory breathlessness accounts for one in eight of all emergency hospitalizations. Early, noninvasive diagnostic testing is a clinical priority that allows rapid triage and treatment. Here, we sought to find replicate breath volatile organic compound (VOC) biomarkers acute disease understand metabolite network enrichment disease, with view gaining mechanistic insight biochemical derangements. We collected analyzed exhaled samples from 277 participants presenting...
Abstract Introduction Many adults hospitalised with COVID-19 have persistent symptoms such as fatigue, breathlessness and brain fog that limit day-to-day activities. These can last over 2 years. Whilst there is limited controlled studies on interventions support those ongoing symptoms, has been some promise in rehabilitation improving function either using face-to-face or digital methods, but evidence remains these often lack a control group. Methods analysis This nested single-blind,...
The risk factors for recovery from COVID-19 dyspnoea are poorly understood. We investigated determinants of in adults with and compared these to non-COVID-19 dyspnoea.
The scale of COVID-19 and its well documented long-term sequelae support a need to understand outcomes including frailty.
Abstract Background The striking increase in COVID-19 severity older adults provides a clear example of immunesenescence, the age-related remodelling immune system. To better characterise association between convalescent immunesenescence and acute disease severity, we determined phenotype survivors non-infected controls. Results We performed detailed phenotyping peripheral blood mononuclear cells isolated from 103 3–5 months post recovery who were classified as having had severe ( n = 56;...
Bronchial thermoplasty is a treatment for asthma. It currently unclear whether its histopathological impact sufficiently explained by the proportion of airway wall that exposed to temperatures necessary affect cell survival.Airway smooth muscle and bronchial epithelial cells were media (37-70°C) 10 s mimic thermoplasty. In silico we developed mathematical model heat distribution post-thermoplasty. vivo determined mass integrity pre- post-thermoplasty in 14 patients with severe asthma.In...