David J. Hughes
A5039013705- Selenium in Biological Systems
- Trace Elements in Health
- Genetic factors in colorectal cancer
- Gut microbiota and health
- Sarcoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Heavy Metal Exposure and Toxicity
- BRCA gene mutations in cancer
- Colorectal Cancer Screening and Detection
- Nutritional Studies and Diet
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- DNA Repair Mechanisms
- Nutrition, Genetics, and Disease
- Vitamin D Research Studies
- Membrane Separation Technologies
- interferon and immune responses
- Diet and metabolism studies
- Genetic Associations and Epidemiology
- Helicobacter pylori-related gastroenterology studies
- Nanopore and Nanochannel Transport Studies
- Advanced Glycation End Products research
- Mycobacterium research and diagnosis
- Cystic Fibrosis Research Advances
- Cardiac tumors and thrombi
University College Dublin
2010-2025
Royal Children's Hospital
2018-2025
University of St Andrews
2019-2025
Sheffield Teaching Hospitals NHS Foundation Trust
2010-2024
Teesside University
2024
University of Sheffield
2010-2023
University of Oxford
2006-2021
Australian Sports Commission
2020
Royal College of Surgeons in Ireland
2012-2017
Weston Park Cancer Centre
2017
In this consensus statement, an international panel of experts deliver their opinions on key questions regarding the contribution human microbiome to carcinogenesis.International in oncology and/or research were approached by personal communication form a panel. A structured, iterative, methodology based around 1-day roundtable discussion was employed derive expert microbiome-oncology research.Some 18 convened for and five identified regarding: (1) relevance dysbiosis/an altered gut...
Suboptimal intakes of the micronutrient selenium (Se) are found in many parts Europe. Low Se status may contribute to colorectal cancer (CRC) development. We assessed by measuring serum levels and Selenoprotein P (SePP) examined association with CRC risk a nested case–control design (966 cases; 966 matched controls) within European Prospective Investigation into Cancer Nutrition. was measured total reflection X‐ray fluorescence SePP immunoluminometric sandwich assay. Multivariable incidence...
Chemotherapy for advanced colorectal cancer leads to improved survival; however, predictors of response systemic treatment are not available. Genomic and epigenetic alterations the gene encoding transcription factor AP-2 epsilon (TFAP2E) common in human cancers. The dickkopf homolog 4 protein (DKK4) is a potential downstream target TFAP2E has been implicated chemotherapy resistance. We aimed further evaluate role DKK4 as chemotherapy.
Low selenium (Se) status correlates with increased risk of colorectal cancer (CRC). Since Se exerts its biological roles through the selenoproteins, genetic variations in selenoprotein genes may influence susceptibility to CRC. This study analysed 12 single-nucleotide polymorphisms (SNPs) [glutathione peroxidase 1 ( GPX1 ), GPX4 , 15 kDa SEP15 S SELS P (SEPP1) and thioredoxin reductase 2 TXNRD2 )] that code for a key protein incorporation [SECIS-binding SBP2 antioxidant defence [superoxide...
Adequate intake of copper and zinc, two essential micronutrients, are important for antioxidant functions. Their imbalance may have implications development diseases like colorectal cancer (CRC), where oxidative stress is thought to be etiologically involved. As evidence from prospective epidemiologic studies lacking, we conducted a case-control study nested within the European Prospective Investigation into Cancer Nutrition (EPIC) cohort investigate association between circulating levels...
AIMTo examine the effect of Fusobacterium nucleatum (F.nucleatum ) on microenvironment colonic neoplasms and expression inflammatory mediators microRNAs (miRNAs). METHODSLevels F. DNA, cytokine gene mRNA (TLR2 , TLR4 NFKB1 TNF IL1B IL6 IL8 ), potentially interacting miRNAs (miR-21-3p, miR-22-3p, miR-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction (qPCR) TaqMan ® assays in DNA and/or RNA extracted from disease adjacent normal fresh tissues 27 colorectal...
Screening for the early detection of colorectal cancer is important to improve patient survival. The aim this study was investigate potential circulating cell-free miRNAs as biomarkers CRC, and their efficiency at delineating patients with polyps benign adenomas from normal groups. expression 667 assessed in a discovery set 48 plasma samples comprising normal, polyp, adenoma, advanced samples. Three (miR-34a, miR-150, miR-923) were further examined validation cohort 97 subjects divided into...
Copper and zinc are essential micronutrients cofactors of many enzymatic reactions that may be involved in liver-cancer development. We aimed to assess pre-diagnostic circulating levels copper, their ratio (Cu/Zn) relation hepatocellular carcinoma (HCC), intrahepatic bile duct (IHBD) gall bladder biliary tract (GBTC) cancers. A nested case–control study was conducted within the European Prospective Investigation into Cancer Nutrition cohort. Serum copper were measured baseline blood samples...
Dietary selenium (Se) intake is essential for synthesizing selenoproteins that are important in countering oxidative and inflammatory processes linked to colorectal carcinogenesis. However, there limited knowledge on the selenoprotein expression adenoma (CRA) cancer (CRC) patients, or interaction with Se status levels. We studied of seventeen pathway genes (including fifteen twenty-five human selenoproteins) RNA extracted from disease-normal tissue pairs, discovery phase sixty-two CRA/CRC...
The gut microbiota is implicated in the pathogenesis of colorectal cancer (CRC). We aimed to map CRC mucosal and metabolome define influence tumoral on oncological outcomes.A multicentre, prospective observational study was conducted patients undergoing primary surgical resection UK (n = 74) Czech Republic 61). Analysis performed using metataxonomics, ultra-performance liquid chromatography-mass spectrometry (UPLC-MS), targeted bacterial qPCR tumour exome sequencing. Hierarchical clustering...
There is increasing evidence indicating a role for Fusobacterium nucleatum (F. nucleatum) in colorectal cancer (CRC) development and prognosis. This study evaluated F. as prognostic biomarker, by assessing its association with post-diagnosis survival from CRC. From September 2008 to April 2012 CRC patients (n = 190) were recruited three hospitals within the Czech Republic. DNA copies measured adjacent non-malignant tumor tissues using quantitative real-time PCR. Cox Proportional Hazards (HR)...
Higher circulating 25-hydroxyvitamin D levels (25(OH)D) have been found to be associated with lower risk for colorectal cancer (CRC) in prospective studies. Whether this association is modified by genetic variation genes related vitamin metabolism and action has not well studied humans. We investigated 1307 functional tagging single-nucleotide polymorphisms (SNPs; individually, gene/pathway) 86 D-related 1420 incident CRC cases matched controls from the European Prospective Investigation...